Impact of elevated temperatures on specific leaf weight, stomatal density, photosynthesis and chlorophyll fluorescence in soybean
Impact of elevated temperatures on specific leaf weight, stomatal density, photosynthesis and chlorophyll fluorescence in soybean
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DOI：
10.1007/s11120-016-0326-y
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发表时间：
2017-03-01
影响因子：
3.7
通讯作者：
Pandey, Govind Prakash
中科院分区：
文献类型：
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作者：
Jumrani, Kanchan;Bhatia, Virender Singh;Pandey, Govind Prakash
高温胁迫是一种主要的环境胁迫，目前关于高温胁迫对大豆影响的研究有限。本研究旨在量化高温对大豆叶片厚度、叶片正面和背面气孔数、气体交换、叶绿素荧光参数和种子产量变化的影响。12个基因型大豆在温室条件下生长，昼夜温度分别为30/22、34/24、38/26和42/28℃，平均温度分别为26、29、32和35℃。其中一组在作物季平均最高、最低和平均温度分别为28.0、22.4和25.2℃的环境温度条件下种植。温度对叶片比重和叶片厚度有显著的负向影响。光合速率、气孔导度和水分利用效率随生长温度的升高而降低；细胞间CO2和蒸腾速率增加。随着温度的升高，叶绿素荧光参数Fv/Fm、qP和PhiPSII下降，qN增加。随着温度的升高，叶片背面和正面气孔数量均显著增加。光合速率、PhiPSII、qP和SPAD值与叶片厚度和SLW呈显著正相关。这表明光合作用和相关参数的减少似乎是由于在较高温度下观察到的结构变化。常温条件下种子产量最高（13.2 g/pl），随着温度升高至30/22℃、34/24℃、38/26℃和42/28℃，种子产量分别下降了8%、14%、51%和65%。
High-temperature stress is a major environmental stress and there are limited studies elucidating its impact on soybean (Glycine max L. Merril.). The objectives of present study were to quantify the effect of high temperature on changes in leaf thickness, number of stomata on adaxial and abaxial leaf surfaces, gas exchange, chlorophyll fluorescence parameters and seed yield in soybean. Twelve soybean genotypes were grown at day/night temperatures of 30/22, 34/24, 38/26 and 42/28 A degrees C with an average temperature of 26, 29, 32 and 35 A degrees C, respectively, under greenhouse conditions. One set was also grown under ambient temperature conditions where crop season average maximum, minimum and mean temperatures were 28.0, 22.4 and 25.2 A degrees C, respectively. Significant negative effect of temperature was observed on specific leaf weight (SLW) and leaf thickness. Rate of photosynthesis, stomatal conductance and water use efficiency declined as the growing temperatures increased; whereas, intercellular CO2 and transpiration rate were increased. With the increase in temperature chlorophyll fluorescence parameters such as Fv/Fm, qP and PhiPSII declined while there was increase in qN. Number of stomata on both abaxial and adaxial surface of leaf increased significantly with increase in temperatures. The rate of photosynthesis, PhiPSII, qP and SPAD values were positively associated with leaf thickness and SLW. This indicated that reduction in photosynthesis and associated parameters appears to be due to structural changes observed at higher temperatures. The average seed yield was maximum (13.2 g/pl) in plants grown under ambient temperature condition and declined by 8, 14, 51 and 65% as the temperature was increased to 30/22, 34/24, 38/26 and 42/28 A degrees C, respectively.