A microtubule-independent role for centrosornes and Aurora A in nuclear envelope breakdown
A microtubule-independent role for centrosornes and Aurora A in nuclear envelope breakdown
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DOI：
10.1016/j.devcel.2007.01.019
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发表时间：
2007-04-01
影响因子：
11.8
通讯作者：
Oegema, Karen
中科院分区：
文献类型：
--
作者：
Portier, Nathan;Audhya, Anjon;Oegema, Karen
Aurora A激酶定位于中心体，是中心体成熟和纺锤体组装所必需的。在这里，我们描述了一个微管无关的作用，极光A和中心体在核膜破裂（NEBD）在第一次有丝分裂的C。线虫胚胎Aurora A耗竭不会改变染色体凝聚的发生或动力学，但显着延长了凝聚完成和NEBD之间的间隔。通过其他方式抑制中心体组装也延长了这一间隔，尽管程度低于Aurora A耗尽。相比之下，中心体有核微管和核小体相关的运动动力蛋白不需要及时NEBD。这些结果表明，有丝分裂中心体产生一个扩散因子，我们建议激活极光A，促进NEBD。一个正反馈环，其中极光A依赖的中心体大小的增加促进极光A激活，可能暂时耦合中心体成熟NEBD在有丝分裂进入。
Aurora A kinase localizes to centrosomes and is required for centrosome maturation and spindle assembly. Here we describe a microtubule-independent role for Aurora A and centrosomes in nuclear envelope breakdown (NEBD) during the first mitotic division of the C. elegans embryo. Aurora A depletion does not alter the onset or kinetics of chromosome condensation, but dramatically: lengthens the interval between the completion of condensation and NEBD. Inhibiting centrosome assembly by other means also lengthens this interval, albeit to a lesser extent than Aurora A depletion. By contrast, centrosomally nucleated microtubules and the nuclear envelope-associated motor dynein are not required for timely NEBD. These results indicate that mitotic centrosomes generate a diffusible factor, which we propose is activated Aurora A, that promotes NEBD. A positive feedback loop, in which an Aurora A-dependent increase in centrosome size promotes Aurora A activation, may temporally couple centrosome maturation to NEBD during mitotic entry.