Multichannel micromanipulator and chamber system for recording multineuronal activity in alert, non-human primates
Multichannel micromanipulator and chamber system for recording multineuronal activity in alert, non-human primates
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DOI：
10.1152/jn.00259.2007
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发表时间：
2007-07-01
影响因子：
2.5
通讯作者：
Lear, Alex
中科院分区：
文献类型：
--
作者：
Gray, Charles M.;Goodell, Baldwin;Lear, Alex
用于记录警觉的非人类灵长类动物多神经元活动的多通道显微操作器和腔室系统。J Neurophysiol 98：527-536，2007.首次发表于2007年5月9日doi：10.1152/jn.00259.2007。我们描述了一个机电系统的设计和性能进行多神经元记录实验警觉非人灵长类动物。该系统基于一个简单的设计，包括一个微驱动器，控制电子设备，软件和一个独特的记录室。微驱动器由铝框架、由计算机控制的微型步进电机驱动的一组八个线性致动器和两个提供电极和控制电子设备连接的印刷电路板（PCB）组成。控制电路围绕基于Atmel RISC的微控制器构建，该微控制器向多达8个电机控制卡发送命令，每个控制卡能够控制8个电机。微控制器用C编程，并使用串行通信与主机连接。用于发送命令的图形用户界面是用C编写的，并在传统的个人计算机上运行。记录室是低的轮廓，安装在一个圆形的开颅手术，并纳入一个可移动的内部套管。可更换的Sylastic膜可以横跨套管的底部开口伸展，以在颅腔和外部环境之间提供水密密封。这大大降低了对感染的易感性，几乎消除了常规清洁的需要，并允许在相同部位将电极重复引入大脑，同时保持防水密封。该系统可靠，易于使用，并且与具有类似功能的其他市售系统相比具有多个优点。
Multichannel micromanipulator and chamber system for recording multineuronal activity in alert, non-human primates. J Neurophysiol 98: 527-536, 2007. First published May 9, 2007 doi: 10.1152/jn.00259.2007. We describe the design and performance of an electromechanical system for conducting multineuron recording experiments in alert non- human primates. The system is based on a simple design, consisting of a microdrive, control electronics, software, and a unique type of recording chamber. The microdrive consists of an aluminum frame, a set of eight linear actuators driven by computer- controlled miniature stepping motors, and two printed circuit boards ( PCBs) that provide connectivity to the electrodes and the control electronics. The control circuitry is structured around an Atmel RISC- based microcontroller, which sends commands to as many as eight motor control cards, each capable of controlling eight motors. The microcontroller is programmed in C and uses serial communication to interface with a host computer. The graphical user interface for sending commands is written in C and runs on a conventional personal computer. The recording chamber is low in profile, mounts within a circular craniotomy, and incorporates a removable internal sleeve. A replaceable Sylastic membrane can be stretched across the bottom opening of the sleeve to provide a watertight seal between the cranial cavity and the external environment. This greatly reduces the susceptibility to infection, nearly eliminates the need for routine cleaning, and permits repeated introduction of electrodes into the brain at the same sites while maintaining the watertight seal. The system is reliable, easy to use, and has several advantages over other commercially available systems with similar capabilities.