Interaction of nuclear protein p140 with human immunodeficiency virus type 1 TAR RNA in mitogen-activated primary human T lymphocytes.
Interaction of nuclear protein p140 with human immunodeficiency virus type 1 TAR RNA in mitogen-activated primary human T lymphocytes.
复制标题
核蛋白 p140 与人免疫缺陷病毒 1 型 TAR RNA 在丝裂原激活的原代人 T 淋巴细胞中的相互作用。
DOI：
10.1128/jvi.69.8.5156-5163.1995
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Kumar,A
中科院分区：
文献类型：
--
作者：
Rothblum,CJ;Jackman,J;Mikovits,J;Shukla,RR;Kumar,A
Several lines of evidence suggest that cellular proteins play a role during human immunodeficiency virus type 1 (HIV-1) Tat-mediated trans activation. A recent report from this laboratory has shown that a 140-kDa HeLa nuclear protein (p140) binds specifically to the lower stem region of the Tat response element, TAR RNA. Since HIV-1 trans activation is most efficient in proliferating T cells, we investigated the binding of p140 to TAR RNA in unstimulated and mitogen-activated, G1-phase primary T lymphocytes. TAR RNA/protein-binding activity was low in resting cells but increased significantly within 2 h of activation and remained elevated for at least 48 h. Corresponding increases in p140 protein levels were observed with most but not all donors, suggesting that an additional nuclear factor(s) may be required for efficient binding of this protein to TAR RNA in activated T cells.
