Isolation of mutant Saccharomyces cerevisiae strains that survive without sphingolipids.
Isolation of mutant Saccharomyces cerevisiae strains that survive without sphingolipids.
复制标题
分离在没有鞘脂的情况下也能存活的突变酿酒酵母菌株。
DOI：
10.1128/mcb.10.5.2176-2181.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
Lester,RL
中科院分区：
文献类型：
--
作者：
Dickson,RC;Wells,GB;Schmidt,A;Lester,RL
鞘脂包括一个大的，广泛的家庭复杂的真核细胞膜成分的功能不明确。酵母Saccharomyces gongaeis特别适合于鞘脂功能的研究，因为它含有少量的鞘脂，并且适合于分子遗传分析。此外，它是唯一的真核生物中的突变体阻断鞘脂生物合成已被分离。从一个nonreverting鞘脂缺陷的菌株，需要添加鞘脂的长链碱基成分的培养基中生长，我们分离出两个菌株携带二级，抑制突变，允许在没有外源性长链碱基的情况下生存。值得注意的是，抑制菌株几乎不产生鞘脂。对抑制基因产物如何补偿鞘脂缺乏的研究可能揭示这些膜脂在酵母细胞中的功能。
Sphingolipids comprise a large, widespread family of complex eucaryotic-membrane constituents of poorly defined function. The yeastSaccharomyces cerevisiaeis particularly suited for studies of sphingolipid function because it contains a small number of sphingolipids and is amenable to molecular genetic analysis. Moreover, it is the only eucaryote in which mutants blocked in sphingolipid biosynthesis have been isolated. Beginning with a nonreverting sphingolipid-defective strain that requires the addition of the long-chain-base component of sphingolipids to the culture medium for growth, we isolated two strains carrying secondary, suppressor mutations that permit survival in the absence of exogenous long-chain base. Remarkably, the suppressor strains made little if any sphingolipid. A study of how the suppressor gene products compensate for the lack of sphingolipids may reveal the function(s) of these membrane lipids in yeast cells.