LEUCINE BIOSYNTHESIS IN YEAST - IDENTIFICATION OF 2 GENES (LEU4, LEU5) THAT AFFECT ALPHA-ISOPROPYLMALATE SYNTHASE ACTIVITY AND EVIDENCE THAT LEU1 AND LEU2 GENE-EXPRESSION IS CONTROLLED BY ALPHA-ISOPROPYLMALATE AND THE PRODUCT OF A REGULATORY GENE
LEUCINE BIOSYNTHESIS IN YEAST - IDENTIFICATION OF 2 GENES (LEU4, LEU5) THAT AFFECT ALPHA-ISOPROPYLMALATE SYNTHASE ACTIVITY AND EVIDENCE THAT LEU1 AND LEU2 GENE-EXPRESSION IS CONTROLLED BY ALPHA-ISOPROPYLMALATE AND THE PRODUCT OF A REGULATORY GENE
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DOI：
10.1007/bf00445877
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发表时间：
1983-01-01
期刊：
影响因子：
2.5
通讯作者：
KOHLHAW, GB
中科院分区：
文献类型：
--
作者：
BAICHWAL, VR;CUNNINGHAM, TS;KOHLHAW, GB
结果表明，酵母α-异丙基苹果酸合酶活性由两个独立的基因决定，分别命名为LEU 4和LEU 5，其中LEU 4为结构基因，LEU 5为另一个结构基因，或为另一个结构基因的表达提供功能。影响亮氨酸生物合成及其调节的突变体的特性表明，LEU 1和LEU 2（分别编码异丙基苹果酸异构酶和β-异丙基苹果酸脱氢酶的结构基因）的表达受到α-异丙基苹果酸和调节元件（LEU 3基因产物）的复合物的控制。讨论了酵母菌和粗糙脉孢菌在亮氨酸生物合成方面的异同。
Tetrad analysis indicates that α-isopropylmalate synthase activity of yeast is determined by two separate genes, designatedLEU4andLEU5.LEU4is identified as a structural gene.LEU5either encodes another α-isopropylmalate synthase activity by itself or provides some function needed for the expression of a second structural gene. The properties of mutants affecting the biosynthesis of leucine and its regulation suggest that the expression ofLEU1andLEU2(structural genes encoding isopropylmalate isomerase andβ-isopropylmalate dehydrogenase, respectively) is controlled by a complex of a-isopropylmalate and a regulatory element (theLEU3gene product). Similarities and differences between yeast andNeurospora crassawith respect to leucine biosynthesis are discussed.