A selection guide for the new generation 6-dye DNA profiling systems
A selection guide for the new generation 6-dye DNA profiling systems
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DOI：
10.1016/j.fsigen.2017.05.010
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发表时间：
2017-09-01
影响因子：
3.1
通讯作者：
Ip, Stephen C. Y.
中科院分区：
文献类型：
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作者：
Lin, Sze-Wah;Li, Christina;Ip, Stephen C. Y.
联邦调查局（FBI）最近将CODIS核心基因座从现有的13个扩大到20个，作为新的指导方针，来自美国的实验室在上传或在国家数据库中进行身份搜索之前必须遵守新的规定。扩展后的CODIS格式共享欧洲国家和美国常用的所有核心基因座，不仅提高了国际兼容性，而且减少了偶然匹配的数量，从而促进了国际执法和反恐努力。在这里，我们回顾了三个新的STR扩增系统的关键性能指标与6染料化学，即调查24倍QS试剂盒从QIAGEN，GlobalFiler（TM）PCR扩增试剂盒从应用生物系统（TM），和PowerFiler（R）融合6C系统从Promega。我们的结果表明，GlobalFiler在测试的试剂盒中显示出最高的灵敏度，而Investigator 24plex对常见的PCR抑制剂（包括腐殖酸和单宁酸）显示出更高的耐受性。另一方面，GlobalFiler和Fusion 6C产生具有更好的杂合峰高和色内信号平衡的DNA图谱。GlobalFiler和Investigator 24 plex在DNA混合物中次要组分的分析中均表现出比Fusion 6C略高的灵敏度，但后者在保持混合比例方面表现出更高的一致性。综上所述，我们的工作表明，这三种分析系统具有不同的性能特点，因此建议实验室应根据自己的要求和操作需要选择最合适的试剂盒。(C)2017爱思唯尔B.V.保留所有权利。
The Federal Bureau of Investigation (FBI) has recently expanded the CODIS core loci from the existing 13 to 20 as a new guideline, and laboratories from the US are required to comply with the new regulation before uploading or conducting identity search in the national database. The expanded CODIS format, which shares all the core loci commonly used in the European countries and the US, not only increases international compatibility, but also reduces the number of adventitious matches, and hence facilitates international law enforcement and counterterrorism endeavours. Here, we review the key performance measures of three new STR amplification systems with 6-dye chemistry, namely, the Investigator 24plex QS Kit from QIAGEN, the GlobalFiler (TM) PCR Amplification Kit from Applied Biosystems (TM), and the PowerPlex (R) Fusion 6C System from Promega. Our results have demonstrated that GlobalFiler displays the highest sensitivity among the tested kits, whereas Investigator 24plex shows a higher tolerance to common PCR inhibitors including Humic acid and Tannic acid. GlobalFiler and Fusion 6C, on the other hand, yield DNA profiles with better heterozygous peak height and intra-colour signal balance. Both GlobalFiler and Investigator 24plex exhibit slightly higher sensitivity than Fusion 6C in the profiling of minor components in DNA mixture, but the latter displays a higher consistency in the preservation of the mixture ratio. In summary, our work has demonstrated that these three profiling systems have their different performance features, and hence it is recommended that laboratories should select the most suitable kits according to their own requirements and operational needs. (C) 2017 Elsevier B.V. All rights reserved.