Peripheral territory and neuropeptides of the trigeminal ganglion neurons centrally projecting through the oculomotor nerve demonstrated by fluorescent retrograde double-labeling combined with immunocytochemistry
Peripheral territory and neuropeptides of the trigeminal ganglion neurons centrally projecting through the oculomotor nerve demonstrated by fluorescent retrograde double-labeling combined with immunocytochemistry
复制标题
DOI：
10.1016/0006-8993(91)90577-i
复制
发表时间：
1991-04
期刊：
影响因子：
2.9
通讯作者：
R. Bortolami;L. Calzà;M. Lucchi;L. Giardino;E. Callegari;E. Manni;V. E. Pettorossi;A. M. Barazzoni;G. L. Costerbosa
中科院分区：
文献类型：
--
作者：
R. Bortolami;L. Calzà;M. Lucchi;L. Giardino;E. Callegari;E. Manni;V. E. Pettorossi;A. M. Barazzoni;G. L. Costerbosa
通过在双标记实验中使用荧光示踪剂来研究绵羊三叉神经元的外周区域，这些神经元通过动眼神经将其中枢过程发送到脑干。为此目的，将二脒黄 (DY) 注射到动眼神经中，并与固蓝 (FB) 注射到眼外肌 (EOM)、角膜或上眼睑中。除了单标记的 DY 或 FB 细胞外，在三叉神经节的眼部区域还发现了双标记的 DY+FB 细胞。对DY和DY+FB标记的三叉神经细胞进行免疫细胞化学分析其P物质(SP)-、降钙素基因相关肽(CGRP)-和胆囊收缩素-8(CCK-8)样的含量。所有单标记 DY 细胞均显示 SP、CGRP 或 CCK-8 样免疫反应性。支配 EOMS 的双标记 DY+FB 神经元对三种肽均具有免疫反应性，而供应角膜的双标记神经元仅呈 CGRP 样阳性。研究结果表明，在绵羊中，三叉神经元通过动眼神经集中发送信号，供应 EOM、角膜和上眼睑，并含有通常与痛觉相关的神经肽。
The peripheral territories of sheep trigeminal neurons which send their central process to the brainstem through the oculomotor nerve were investigated by the use of fluorescent tracers in double-labeling experiments. For this purpose Diamidino yellow (DY) injection into the oculomotor nerve was combined with Fast blue (FB) injection either into the extraocular muscles (EOMs), or the cornea, or the superior eyelid. Double-labeled DY+FB cells were found in the opthalmic region of the trigeminal ganglion in addition to single-labeled DY or FB cells. The DY and DY+FB-labeled trigeminal cells were analysed immunocytochemically for their content of subtance P (SP)-, calcitonin gene-related peptide (CGRP)-, and cholecystokinin-8 (CCK-8)-like. All single-labeled DY cells showed SP-, CGRP- or CCK-8-like immunoreactivity. Double-labeled DY+FB neurons innervating the EOMS were immunoreactive for each of the three peptides, whereas double-labeled neurons supplying the cornea were only CGRP-like positive. The findings suggest that, in the sheep, trigeminal neurons which send their process centrally through the oculomotor nerve supply the EOMs, the cornea, and the superior eyelid and contain neuropeptides which are usually associated with pain sensation.