Whole body vibration improves osseointegration by up-regulating osteoblastic activity but down-regulating osteoblast-mediated osteoclastogenesis via ERK1/2 pathway
Whole body vibration improves osseointegration by up-regulating osteoblastic activity but down-regulating osteoblast-mediated osteoclastogenesis via ERK1/2 pathway
复制标题
全身振动通过 ERK1/2 途径上调成骨细胞活性，但下调成骨细胞介导的破骨细胞生成，从而改善骨整合
DOI：
10.1016/j.bone.2014.09.026
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发表时间：
2015
期刊：
影响因子：
4.1
通讯作者：
Wang Huiming
中科院分区：
文献类型：
--
作者：
Zhou Yi;Guan Xiaoxu;Liu Tie;Wang Xinhua;Yu Mengfei;Yang Guoli;Wang Huiming
由于骨量减少和骨微结构恶化，骨质疏松症是影响种植体骨结合的重要危险因素。最近，低幅高频（LMHF）振动（LM：< 1 ×g; HF：20-90 Hz）已被证明对骨骼稳态具有合成代谢但抗吸收作用。因此，我们假设LMHF载荷，在全身振动（WBV）方面，可能会改善骨关节炎状态下的种植体固定。在体内研究中，将羟基磷灰石涂层钛种植体植入卵巢切除大鼠双侧胫骨后，应用WBV治疗（幅度：0.3 g，频率：40 Hz，时间：30 min/12 h，5天/周）。植入12周后测量骨质量和骨特异性基因表达。在体外研究中，使用各种实验测定充分研究了成骨细胞和骨诱导活性的细胞和分子机制。Micro-CT检查显示，WBV可以通过改善种植体周围的微观结构参数来促进骨结合。WBV调控的基因水平有利于骨形成而不是骨吸收，这可能是骨-种植体表面良好的适应性骨重塑的原因。体外实验结果表明，振动（振幅：0.3g，频率：40 Hz，时间：30 min/12 h）促进成骨细胞分化、基质合成和矿化。然而，机械调节的成骨细胞活性主要是通过影响成骨细胞产生破骨细胞生成相关的关键可溶性因子，包括RANKL和M-CSF。因此，成骨细胞是机械力转导过程中的直接靶细胞。ERK 1/2信号通路在振动诱导的骨形成增强和骨吸收减少中起重要作用。我们的数据表明，WBV是一种有益的非药物干预，以改善骨质疏松症下的骨整合。
Due to the reduction in bone mass and deterioration in bone microarchitecture, osteoporosis is an important risk factor for impairing implant osseointegration. Recently, low-magnitude, high-frequency (LMHF) vibration (LM: < 1 ×g; HF: 20–90 Hz) has been shown to exhibit anabolic, but anti-resorptive effects on skeletal homeostasis. Therefore, we hypothesized that LMHF loading, in terms of whole body vibration (WBV), may improve implant fixation under osteoporotic status. In thein vivostudy, WBV treatment (magnitude: 0.3 g, frequency: 40 Hz, time: 30 min/12 h, 5 days/week) was applied after hydroxyapatite-coated titanium implants were inserted in the bilateral tibiae of ovariectomized rats. The bone mass and the osteospecific gene expressions were measured at 12 weeks post implantation. In thein vitrostudy, the cellular and molecular mechanisms underlying osteoblastic and osteoclastic activities were fully investigated using various experimental assays. Micro-CT examination showed that WBV could enhance osseointegration by improving microstructure parameters surrounding implants. WBV-regulated gene levels in favor of bone formation over resorption may be the reason for the favorable adaptive bone remolding on bone-implant surface. Thein vitrostudy showed that vibration (magnitude: 0.3 g, frequency: 40 Hz, time: 30 min/12 h) up-regulated osteoblast differentiation, matrix synthesis and mineralization. However, mechanically regulated osteoclastic activity was mainly through the effect on osteoblastic cells producing osteoclastogenesis-associated key soluble factors, including RANKL and M-CSF. Osteoblasts were therefore the direct target cells during the mechanotransduction process. The ERK1/2 pathway was demonstrated to play an essential role in vibration-induced enhancement of bone formation and decreased bone resorption. Our data suggests that WBV was a helpful non-pharmacological intervention for improving osseointegration under osteoporosis.