Bacterial communities in Asian dust-containing snow layers on Mt. Tateyama, Japan
Bacterial communities in Asian dust-containing snow layers on Mt. Tateyama, Japan
复制标题
DOI：
10.5331/bgr.29.31
复制
发表时间：
2011
影响因子：
1
通讯作者：
D. Tanaka;Yumi Tokuyama;Yohei Terada;Kohta Kunimochi;Chika Mizumaki;Syoko Tamura;M. Wakabayashi;Kazuma Aoki;W. Shimada;Hitoshi Tanaka;S. Nakamura
中科院分区：
文献类型：
--
作者：
D. Tanaka;Yumi Tokuyama;Yohei Terada;Kohta Kunimochi;Chika Mizumaki;Syoko Tamura;M. Wakabayashi;Kazuma Aoki;W. Shimada;Hitoshi Tanaka;S. Nakamura
来自亚洲沙漠地区的亚洲沙尘颗粒被困在日本立山山西部高原的春雪中。2008年和2009年，我们调查了含亚洲尘埃的雪层和其他下垫雪层中的细菌群落。从亚洲含尘雪层中分离出的细菌中存在几个属，包括假定与粉尘相关的细菌芽孢杆菌属。特别是，我们连续2年检测到具有相同16S rDNA序列的分离株（AB500941），这些分离株与枯草芽孢杆菌关系最密切。pcr -变性梯度凝胶电泳（DGGE）分析表明，2年来亚洲含尘雪层细菌群落特征相似。细菌群落在亚洲含尘层和其他层之间存在差异。细菌细胞总数从6.85×10²到2.39×105 cells ml-1不等，亚洲含尘层的细菌细胞总数高于其他层。反向轨迹分析显示，每年亚洲尘埃的可能来源都是相似的。亚洲含尘层的细菌群落组成可能反映了来自尘源地区的微生物的远距离迁移。
Asian dust particles transported from Asian desert areas are trapped in spring snow on the western plateau of Mt. Tateyama, Japan. We investigated bacterial communities in snow layers containing Asian dust, and other underlying snow layers, on Mt. Tateyama during 2008 and 2009. Several genera of bacteria were present among isolates obtained from Asian dust-containing snow layers, including the presumed dust-associated bacterial genus Bacillus. In particular, we detected isolates with identical 16S rDNA sequences (AB500941) over 2 consecutive years, and these isolates were most closely related to Bacillus subtilis. PCR-denaturing gradient gel electrophoresis (DGGE) analysis showed that bacterial community profiles in the Asian dust-containing snow layers were similar over the 2 years. The bacterial communities differed between the Asian dust-containing layers and other layers. Total bacterial cell counts ranged from 6.85×10² to 2.39×105 cells ml-1, with higher values in the Asian dust-containing layers than in the other layers. A backward trajectory analysis showed that the possible source of Asian dust was similar each year. The bacterial community composition of the Asian dust-containing layers presumably reflected the long-distance transportation of microorganisms from dust source regions.