Blood Group Antigen Shielding Facilitated by Selective Cell Surface Engineering
Blood Group Antigen Shielding Facilitated by Selective Cell Surface Engineering
复制标题
选择性细胞表面工程促进血型抗原屏蔽
DOI：
10.1021/acsami.0c00914
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发表时间：
2020
影响因子：
9.5
通讯作者：
Xiantao Shen
中科院分区：
文献类型：
--
作者：
Long Jiang;Huajing Liu;Chuixiu Huang;Xiantao Shen
生产不具有血型抗原免疫原性的红细胞(RBC)在临床化学输血治疗中具有特殊的意义。本研究发展了一种基于分子印迹的选择性细胞表面工程方法，用于制备抗原屏蔽的红细胞。利用表位印迹方法，制备了与红细胞血型抗原具有高亲和力的生物相容性分子印迹纳米凝胶(MIgels)。抗原屏蔽型红细胞可以避免因血型不匹配而引起的凝集，使抗原屏蔽型红细胞在血液供应不足的情况下能够有效地替代红细胞。此外，抗原屏蔽的红细胞能够保持原始红细胞的正常生理结构和功能。我们相信，这项工作中提出的选择性细胞表面工程可能会在生物医学应用的特定细胞保护方面提供显著的好处。
Production of red blood cells (RBCs) without immunogenicity of blood group antigens is of special interest in blood transfusion therapy in clinical chemistry. In this study, a selective cell surface engineering method was developed for the preparation of antigen-shielded RBCs based on molecular imprinting. Using an epitope imprinting method, biocompatible molecularly imprinted nanogels (MIgels) were prepared with a high affinity to the blood group antigens of RBCs. The antigen-shielded RBCs could avoid the agglutination caused by blood group mismatch, resulting in the antigen-shielded RBCs in efficiently substituting RBCs in case of a shortage of blood supply. Moreover, the antigen-shielded RBCs could maintain the normal physiological structure and functions of the original RBCs. We believe that the selective cell surface engineering presented in this work may offer significant benefits in specific cell protection for biomedical application.