Application of a reverse line blot assay to the study of haemoparasites in cattle in Uganda
Application of a reverse line blot assay to the study of haemoparasites in cattle in Uganda
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DOI：
10.1016/j.ijpara.2003.12.012
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发表时间：
2004-04-01
影响因子：
4
通讯作者：
Tait, A
中科院分区：
文献类型：
--
作者：
Oura, CAL;Bishop, RP;Tait, A
基因组技术的最新进展使许多兽医研究人员关注生产一种多价重组疫苗的可能性，以对抗感染热带地区牛的所有血液寄生虫。在开发这种疫苗之前，必须确定目标牛群以及为控制疾病所需的抗病原体疫苗的范围。为了进一步实现这一目标，我们评估了一种反向线印迹（RLB）检测方法，该方法在乌干达不同的流行病学情景中同时检测主要的蜱传原生动物和立克次体牛病原体。一个关键的问题是灵敏度，特别是在检测携带动物方面。由于细小芽孢杆菌被认为是该地区最重要的病原体，我们评估了RLB检测方法对细小芽孢杆菌的敏感性，结果显示每毫升血液中可以检测到1-2 × 10(3)个寄生虫，这一水平与以前开发的PCR方法相当，远低于传统的显微镜检测。我们应用RLB法评估了杂交牛和本地牛之间的病原体谱的差异，结果表明存在不同的谱，与杂交牛的高流行率相比，本地牛的小t型绦虫和牛头绦虫的流行率较低。相比之下，我们发现与杂交牛相比，土种牛中变异芽孢杆菌和白芽孢杆菌的流行率更高。有趣的是，边缘无原体、牛巴贝斯虫和双巴贝斯虫的患病率较低，但牛埃利希体的患病率却很高，这就提出了这种立克次体是否能在牛中致病的问题。对出现东海岸热临床症状的动物的分析表明，细小T是引起这些症状的主要原因，而变形T以及可能的牛头T和利氏T也可能引起临床疾病。总的来说，这里提出的结果突出了乌干达牛中蜱传病原体感染的复杂性。(C) 2004澳大利亚寄生虫学学会Elsevier Ltd.出版。版权所有。
Recent advances in genomic technology have focused many veterinary researchers on the possibility of producing one multivalent recombinant vaccine against all the haemoparasites that infect cattle in the tropics. Before Such a vaccine is developed it is essential to define target cattle Populations as well as the range of anti-pathogen vaccines required in order to control disease. To further this objective, we have evaluated a reverse line blot (RLB) assay, which simultaneously detects the principal tick transmitted protozoan and rickettsial cattle pathogens, in different epidemiological scenarios in Uganda. A critical question is the sensitivity, particularly in relation to detecting carrier animals. As Theileria parva is considered to be the most important pathogen in the region, we assessed the sensitivity of the RLB assay for T. parva and showed that 1-2 X 10(3) parasites per ml of blood could be detected-a level comparable with previously developed PCR methods and well below conventional microscopic detection. We applied the RLB assay to evaluate the differences in pathogen profiles between crossbred and indigenous cattle and show that there were different profiles, with a low prevalence of T. parva and Theileria taurotragi in the indigenous cattle compared to a high prevalence in the crossbred cattle. In contrast, we show higher prevalences of Theileria mutans and Theileria velifera in the indigenous compared to the crossbred cattle. Interestingly Anaplasma marginale, Babesia bovis and Babesia bigemina were of low prevalence but a high prevalence of Ehrlichia bovis was seen, raising the question of whether this rickettsial species could be pathogenic in cattle. Analysis of animals with clinical symptoms of East Coast Fever showed that, while T. parva is a major cause of these symptoms, T. mutans and possibly T taurotragi and T velifiera, may also cause clinical disease. Overall, the results presented here highlight the complexity of tick-borne pathogen infections in cattle in Uganda. (C) 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.