Sec14l3 is specifically expressed in mouse airway ciliated cells
Sec14l3 is specifically expressed in mouse airway ciliated cells
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Sec14l3 在小鼠气道纤毛细胞中特异性表达
DOI：
10.1007/s10753-011-9363-z
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发表时间：
2012
期刊：
影响因子：
5.1
通讯作者：
T.Kawakami
中科院分区：
文献类型：
--
作者：
L.Shan;S.Noritake;M.Fujiwara;S.Asano;C.Yoshida-Noro;N.Noro;K.Yamashita;T.Kawakami
气道上皮是气道完整性的关键组成部分。以前，我们发现，Sec 14 l3基因编码的45 kDa分泌蛋白的表达与实验诱导的气道炎症和肺泡上皮变性/坏死的进展呈负相关。本研究利用原位杂交技术证实小鼠肺纤毛细胞选择性表达Sec 14 l3 mRNA。在小鼠气管上皮细胞的三维培养中，Sec 14 l3 mRNA的水平与纤毛细胞的分化相关。鼻内感染流感病毒的成年小鼠在感染后10天内导致Sec 14 l3 mRNA表达下降20倍。这些结果增强了Sec 14 l3作为纤毛上皮细胞特异性生物标志物的潜在价值，用于气道炎症如气道病毒感染和哮喘的进展。
Airway epithelium is a key component for airway integrity. Previously, we found that expression of theSec14l3gene that encodes a 45-kDa secretory protein is inversely associated with the progression of experimentally induced airway inflammation and degeneration/necrosis of alveolar epithelium. In this report, usingin situhybridization we demonstrated that the ciliated cells in mouse lung selectively expressSec14l3mRNA. In a three-dimensional culture of mouse tracheal epithelial cells, levels of theSec14l3mRNA correlated with the differentiation of ciliated cells. Intranasal infection of adult mice with influenza virus resulted in a 20-fold, progressive decrease inSec14l3mRNA expression over 10 days post infection. These results enhance the potential value ofSec14l3as a ciliated epithelial cell-specific biomarker for the progression of airway inflammations such as airway viral infection and asthma.