Anaerolinea thermolimosa sp nov., Levilinea saccharolytica gen. nov., sp nov and Leptolinea tardivitalis gen. nov., so. nov., novel filamentous anaerobes, and description of the new classes anaerolineae classis nov and Caldilineae classis nov in the bacterial phylum Chloroflexi
Anaerolinea thermolimosa sp nov., Levilinea saccharolytica gen. nov., sp nov and Leptolinea tardivitalis gen. nov., so. nov., novel filamentous anaerobes, and description of the new classes anaerolineae classis nov and Caldilineae classis nov in the bacterial phylum Chloroflexi
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DOI：
10.1099/ijs.0.64169-0
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发表时间：
2006-06-01
影响因子：
2.8
通讯作者：
Kamagata, Yoichi
中科院分区：
文献类型：
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作者：
Yamada, Takeshi;Sekiguchi, Yuji;Kamagata, Yoichi
其中一个嗜热性(IMO-1(T)菌株)和两个中嗜性(Kibi-1(T)和YMTK-2(T)菌株)不形成孢子、不能运动、革兰氏阴性、多细胞丝状微器官综合征，它们以前被分离出来，暂定为Chloroflei门的AnAerolineae类成员。所有的分离物都是严格意义上的厌氧微生物。3株丝状菌的长度均大于100微米，宽度分别为0.3~0.4微米、0.4微米、0.4微米和0.2微米。菌株IMO-1(T)能在pH 6.0~7.5范围内生长，最适生长pH为7.0。菌株IMO-1(T)的最适生长温度为50摄氏度左右(生长在42至55摄氏度之间)。中温菌株Kibi-1(T)和YMTK-2(T)在pH 6.0-7.2范围内生长，最适生长pH为7.0。这两株中温菌株都能在25-50摄氏度的温度范围内生长，最适生长温度在37摄氏度左右。在酵母膏存在下，所有菌株都能与多种碳水化合物一起生长。菌株IMO-1(T)、Kibi-1(T)和YMTK-2(T)的DNA G+C含量分别为53.3%、59.5%和48.2%。高温菌株IMO-1(T)的主要脂肪酸组成为Anteiso-C-17：0、iso-C-15：0、C-16：0和Anteiso-C-15：0，而中温菌株Kibi-1(T)和YMTK-2(T)的脂肪酸组成分别为支链C-14：0、iso-C-15：0、C-16：0和支链C-17：0，支链C-17：0、C-16：0、C-14：0和C(17：)0。根据它们的16S rRNA基因序列进行的详细的系统发育分析表明，这些分离物属于细菌门Chloroflei的类级分类群AnAerolineae，长期以来一直被认为是一个典型的未培养克隆簇。它们的形态、生理、化学分类和遗传特征有力地支持了这一结论，即这些菌株应被描述为绿藻门中三个独立的新类群。在这里，温带无气藻AnAerolina thermolimosa sp.11月(模式菌株IMO-1(T)=JCM 12577(T)=Dsm 16554(T))；Nov.，sp.11月模式菌株Kibi-1(T)=Jcm 12578(T)=Dsm 16555(T)；Nov.，sp.11月提出了模式菌株YMTK-2(T)=JCM 12579(T)=DSM 16556(T)。此外，我们正式建议将暂定的类级分类群‘AnAerolineae’细分为AnAerolineae classis nov.和Caldiineae classis nov.我们还提出了隶属分类群AnAerolineales Order。Nov.，Caldiineales Order.Nov.、无芒雀形科AnAerolineaceae fam.11月和金银花科(Caldiineaceae Fam.)11月
One thermophilic (strain IMO-1(T)) and two mesophilic (strains KIBI-1(T) and YMTK-2(T)) non-spore-forming, non-motile, Gram-negative, multicellular filamentous micro-organ isms, which were previously isolated as members of the tentatively named class 'Anaerolineae' of the phylum Chloroflexi, were characterized. All isolates were strictly anaerobic micro-organisms. The length of the three filamentous isolates was greater than 100 mu m and the width was 0.3-0.4 mu m for strain IMO-1(T), 0.4-0.5 mu m for strain KIBI-1(T) and thinner than 0.2 mu m for strain YMTK-2(T). Strain IMO-1(T) could grow at pH 6.0-7.5 (optimum growth at pH 7.0). The optimal temperature for growth of strain IMO-1(T) was around 50 degrees C (growth occurred between 42 and 55 degrees C). Growth of the mesophilic strains KIBI-1(T) and YMTK-2(T) occurred at pH 6.0-7.2 with optimal growth at pH 7.0. Both of the mesophilic strains were able to grow in a temperature range of 25-50 degrees C with optimal growth at around 37 degrees C. Yeast extract was required for growth of all three strains. All the strains could grow with a number of carbohydrates in the presence of yeast extract. The G + C contents of the DNA of strains IMO-1(T), KIBI-1(T) and YMTK-2(T) were respectively 53.3, 59.5 and 48.2 mol%. Major fatty acids for thermophilic strain IMO-1(T) were anteiso-C-17:0, iso-C-15:0, C-16:0 and anteiso-C-15:0, whereas those for mesophilic strains KIBI-1(T) and YMTK-2(T) were branched C-14:0, iso-C-15:0, C-16:0 and branched C-17:0, and branched C-17:0, C-16:0, C-14:0 and C(17:)0, respectively. Detailed phylogenetic analyses based on their 16S rRNA gene sequences indicated that the isolates belong to the class-level taxon 'Anaerolineae' of the bacterial phylum Chloroflexi, which for a long time had been considered as a typical uncultured clone cluster. Their morphological, physiological, chemotaxonomic and genetic traits strongly support the conclusion that these strains should be described as three novel independent taxa in the phylum Chloroflexi. Here, Anaerolinea thermolimosa sp. nov. (type strain IMO-1(T)=JCM 12577(T)=DSM 16554(T)), Levilinea saccharolytica gen. nov., sp. nov. (type strain KIBI-1(T)=JCM 12578(T) =DSM 16555(T)) and Leptolinea tardivitalis gen. nov., sp. nov. (type strain YMTK-2(T)= JCM 12579(T) = DSM 16556(T)) are proposed. In addition, we formally propose to subdivide the tentative class-level taxon 'Anaerolineae' into Anaerolineae classis nov. and Caldilineae classis nov. We also propose the subordinate taxa Anaerolineales ord. nov., Caldilineales ord. nov., Anaerolineaceae fam. nov. and Caldilineaceae fam. nov.