RAPD and ISSR fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (Arachis hypogaea) cultivars and wild species
RAPD and ISSR fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (Arachis hypogaea) cultivars and wild species
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DOI：
10.1139/gen-44-5-763
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发表时间：
2001-10-01
期刊：
影响因子：
3.1
通讯作者：
Devarumath, RM
中科院分区：
文献类型：
--
作者：
Raina, SN;Rani, V;Devarumath, RM
利用21个随机引物和29个SSR引物对栽培花生（Arachishypogaea，2n = 4x = 40）的亚种间和品种间的遗传变异和亲缘关系以及栽培花生与野生花生的亲缘关系进行了研究。结果表明，随机引物和SSR引物分别在13份花生种质的220个和124个遗传位点上检测到42.7%和54.4%的多态性。此外，基于RAPD，ISSR和RAPD + ISSR数据的树状图精确地组织了两个亚种的5个植物品种分为5个簇。鉴定出一对SSR引物，该引物可以区分同一品种内所有的供试材料。虽然两种标记的多态性指数在0.1 ~ 0.5之间变化，但RAPD引物的多态性指数（0.35-4.65）明显高于SSR引物（0.35-1.73）。利用RAPD和（或）ISSR指纹图谱可以对不同来源的材料进行鉴别。在此基础上，利用分子标记对A.似乎有可能是缺氧。没有486个RAPD和330个ISSR扩增产物被发现是共同的13种花生节和一个物种的每一个部分Heteranthae，Rhizomatosae，和Procumbentes。RAPD、ISSR和RAPD + ISSR数据构建的树状图显示了总体相似的拓扑结构。它们可以分为四组，对应于四个分类学部分中的物种。本研究结果有力地支持了山花生Arachis monticola（2n = 4x = 40）和花生A. hypogaea关系密切，表明A. villosa和A. ipaensis是这些四倍体物种的二倍体野生祖先。
Twenty-one random and 29 SSR primers were used to assess genetic variation and interrelationships among subspecies and botanical varieties of cultivated peanut, Arachis hypogaea (2n = 4x = 40), and phylogenetic relationships among cultivated peanut and wild species of the genus Arachis. In contrast with the previous generalization that peanut accessions lack genetic variation, both random and SSR primers revealed 42.7 and 54.4% polymorphism, respectively, among 220 and 124 genetic loci amplified from 13 accessions. Moreover, the dendrograms based on RAPD, ISSR, and RAPD + ISSR data precisely organized the five botanical varieties of the two subspecies into five clusters. One SSR primer was identified that could distinguish all the accessions analysed within a variety. Although the polymorphic index content varied from 0.1 to 0.5 for both ISSR and RAPD markers, primer index values were substantially higher for RAPD primers (0.35-4.65) than for SSR primers (0.35-1.73). It was possible to identify accessions, particularly those of divergent origins, by RAPD and (or) ISSR fingerprints. Based on these results, marker-based genetic improvement in A. hypogaea appears possible. None of the 486 RAPD and 330 ISSR amplification products were found to be commonly shared among 13 species of section Arachis and one species each of sections Heteranthae, Rhizomatosae, and Procumbentes. Dendrograms constructed from RAPD, ISSR, and RAPD + ISSR data showed overall similar topologies. They could be resolved into four groups corresponding to the species grouped in four taxonomic sections. The present results strongly support the view that Arachis monticola (2n = 4x = 40) and A. hypogaea are very closely related, and indicate that A. villosa and A. ipaensis are the diploid wild progenitors of these tetraploid species.