Virologic and serologic identification of minute virus of canines (canine parvovirus type 1) from dogs in Japan
Virologic and serologic identification of minute virus of canines (canine parvovirus type 1) from dogs in Japan
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DOI：
10.1128/jcm.40.11.3993-3998.2002
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发表时间：
2002-11-01
影响因子：
9.4
通讯作者：
Carmichael, LE
中科院分区：
文献类型：
--
作者：
Mochizuki, M;Hashimoto, M;Carmichael, LE
犬细小病毒（MVC），也称为犬细小病毒1型，最初被认为是一种非致病性因子，因为它是在20世纪60年代末首次从犬粪便标本中分离出来的。然而，随后的病理学和流行病学研究表明，MVC是新生幼犬的病原体，并广泛分布于美国的家犬中。该病毒也被证明会导致胎儿死亡。然而，直到最近才在美国以外的犬中检测到该病毒，可能是因为缺乏用于MVC分离的唯一易感犬细胞系WRCC/3873 D的广泛可用性。应用PCR技术对346只犬的470份临床标本进行了检测，从4只病犬中检出了MVC特异性基因片段（阳性率为1.2%）。使用WRCC/3873 D和MDCK细胞从3份PCR阳性直肠标本中回收病毒。分离株具有与美国参考菌株GA 3相似的抗原和基因组特性，并被鉴定为MVC。此外，血清流行病学证据表明，5.0%的狗具有抗MVC抗体，也表明在日本的狗中存在MVC感染。根据这项研究和最近几份描述MVC现场病例的欧洲报告，很明显，MVC分布在世界各地的家犬中。
Minute virus of canines (MVC), also known as canine parvovirus type 1, was initially believed to be a nonpathogenic agent, since it was first isolated from canine fecal specimens in the late 1960s. However, subsequent pathological as well as epidemiological studies suggested that MVC is a pathogen of neonatal puppies and is widely distributed among domestic dogs in the United States. The virus also has been shown to cause fetal deaths. Nevertheless, the virus was not detected in dogs outside the United States until recently, presumably because of a lack of widespread availability of the only susceptible canine cell line, WRCC/3873D, used for MVC isolation. We examined 470 clinical specimens from 346 dogs by PCR and detected MVC-specific gene fragments from four diseased puppies (positive rate, 1.2%). Viruses were recovered from three PCR-positive rectal specimens by using WRCC/3873D and MDCK cells. The isolates possessed antigenic and genomic properties similar to those of the U.S. reference strain GA3 and were identified as MVC. In addition, seroepidemiological evidence that 5.0% of dogs possessed anti-MVC antibodies also indicated the presence of MVC infection among dogs in Japan. From this study and several recent European reports describing MVC field cases, it is evident that MVC is distributed among domestic dogs worldwide.