HLA- DR typing in polyarticular juvenile idiopathic arthritis: a study from a tertiary care hospital in northern India
HLA- DR typing in polyarticular juvenile idiopathic arthritis: a study from a tertiary care hospital in northern India
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DOI：
10.1111/1756-185x.12198
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发表时间：
2014-03-01
影响因子：
2.5
通讯作者：
Pramod Kumar, Verma
中科院分区：
文献类型：
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作者：
Dibya Ranjan, Behera;Nand Kumar, Singh;Pramod Kumar, Verma
人类白细胞抗原（HLA）与幼年型特发性关节炎（JIA）相关性的研究结果相互矛盾，这可能与种族差异有关。此外，在印度，对JIA，特别是多关节JIA的HLA-DR分型的研究是缺乏的。我们的研究的目的是揭示在北方印度的多关节JIA的队列HLA DR类型的频率。MethodsFifty-two多关节JIA患者包括根据最近的国际联盟协会风湿病分类，2001年。应用序列特异性引物（SSP）聚合酶链反应（PCR）技术对21例患者（18例RF+，3例RF-）进行HLA DR分型。结果HLA-DR 4在RF+多关节型JIA患者中阳性率为23%（5/23），而在对照组中阳性率为4.3%（1/23），相对危险度为5.47，但与RF+多关节型JIA患者相比，HLA-DR 4阳性率明显增高（27.7%比4.43%; P
IntroductionMany studies of human leukocyte antigen (HLA) association with juvenile idiopathic arthritis (JIA) have reported conflicting results, which were probably related to ethnic differences. Moreover, in India, studies on HLA-DR typing on JIA, particularly polyarticular JIA, is lacking.ObjectiveThe aim of our study was to reveal the frequency of HLA DR types in a cohort of polyarticular JIA in northern India.MethodsFifty-two polyarticular JIA patients were included as per the recent International League of Associations for Rheumatology classification, 2001. HLA-DR typing was performed in 21 patients (18 rheumatoid factor [RF]+ and three RF-) by a DNA-based polymerase chain reaction method for the determination of HLA alleles using sequence specific primers (SSP). The results were compared with that of 23 healthy controls of the same age and sex.ResultsHLA-DR4 was present in five cases (23%) in the diseased group while only in one case (4.3%) in the control group with a relative risk of 5.47, but when compared with only RF+ polyarticular JIA, HLA-DR4 was found to be significantly high (27.7% vs. 4.43%; P