Image Analysis Techniques for In Vivo Quantification of Cerebrospinal Fluid Flow.
Image Analysis Techniques for In Vivo Quantification of Cerebrospinal Fluid Flow.
复制标题
脑脊液流体内定量的图像分析技术。
DOI：
10.1101/2023.07.20.549937
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Tithof,Jeffrey
中科院分区：
文献类型：
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作者：
Kim,Daehyun;Gan,Yiming;Nedergaard,Maiken;Kelley,DouglasH;Tithof,Jeffrey
在过去的十年中，人们对了解脑脊液 (CSF) 流动的神经生理学的兴趣大大增加，脑脊液流动在清除大脑中的代谢废物方面发挥着至关重要的作用。这种日益增长的兴趣主要是由两个重大发现引发的：类淋巴系统（大脑深处的间质液与大脑周围的脑脊液之间的溶质交换途径）和脑膜淋巴管（大脑周围组织层中排出脑脊液的淋巴管）。这两个脑脊液系统协同工作，它们的破坏与多种神经系统疾病有关，包括阿尔茨海默病、中风和创伤性脑损伤。在这里，我们提出了通过直接成像注射到脑脊液中的荧光微球来对脑脊液流量进行体内定量的实验技术。我们讨论详细的图像处理方法，包括静止颗粒的配准和掩蔽，以提高测量质量。我们提供通过颗粒追踪量化脑脊液流量的指导，并提供优化流程的技巧。此外，我们描述了测量动脉直径变化的技术，这是一种假设的脑脊液泵送机制。最后，我们概述了如何将这些相同的技术应用于颈部淋巴管，颈部淋巴管收集脑膜淋巴管下游的液体。我们预计这些流体机械技术将对于未来旨在了解脑脊液运输和破坏机制的定量研究以及其他复杂的生物物理系统有价值。
Over the past decade, there has been a tremendously increased interest in understanding the neurophysiology of cerebrospinal fluid (CSF) flow, which plays a crucial role in clearing metabolic waste from the brain. This growing interest was largely initiated by two significant discoveries: the glymphatic system (a pathway for solute exchange between interstitial fluid deep within the brain and the CSF surrounding the brain) and meningeal lymphatic vessels (lymphatic vessels in the layer of tissue surrounding the brain that drains CSF). These two CSF systems work in unison, and their disruption has been implicated in several neurological disorders including Alzheimer’s disease, stroke, and traumatic brain injury. Here, we present experimental techniques forin vivoquantification of CSF flow via direct imaging of fluorescent microspheres injected into the CSF. We discuss detailed image processing methods, including registration and masking of stagnant particles, to improve the quality of measurements. We provide guidance for quantifying CSF flow through particle tracking and offer tips for optimizing the process. Additionally, we describe techniques for measuring changes in arterial diameter, which is an hypothesized CSF pumping mechanism. Finally, we outline how these same techniques can be applied to cervical lymphatic vessels, which collect fluid downstream from meningeal lymphatic vessels. We anticipate that these fluid mechanical techniques will prove valuable for future quantitative studies aimed at understanding mechanisms of CSF transport and disruption, as well as for other complex biophysical systems.