Transcriptional network controlled by the trithorax-group gene ash2 in Drosophila melanogaster
Transcriptional network controlled by the trithorax-group gene ash2 in Drosophila melanogaster
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DOI：
10.1073/pnas.0538075100
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发表时间：
2003-03-18
影响因子：
11.1
通讯作者：
Corominas, M
中科院分区：
文献类型：
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作者：
Beltran, S;Blanco, E;Corominas, M
转录因子缺失、小或同源盘2 （ash2）基因是同源基因阳性调节因子三胸组的一员。ash2的突变等位基因对幼虫/蛹是致命的，并表现出想象中的椎间盘和大脑异常。本研究中使用的等位基因是三胸功能的真正突变，缺乏野生型苍蝇中最长的转录本。为了鉴定ash2的基因靶点，我们使用cDNA微阵列进行了表达分析。参与细胞周期、细胞增殖和细胞粘附的基因是这些靶标之一，其中一些基因已被功能和表达研究证实。尽管三胸蛋白通过调节特定染色体位置的染色质结构起作用，但尚未发现ash2调节基因物理聚集的证据。这项工作代表了第一个微阵列分析，据我们所知，三胸组基因。
The transcription factor absent, small, or homeotic discs 2 (ash2) gene is a member of the trithorax group of positive regulators of homeotic genes. Mutant alleles for ash2 are larval/pupal lethals and display imaginal disc and brain abnormalities. The allele used in this study is a true mutant for the trithorax function and lacks the longest transcript present in wild-type flies. In an attempt to identify gene targets of ash2, we have performed an expression analysis by using cDNA microarrays. Genes involved in cell cycle, cell proliferation, and cell adhesion are among these targets, and some of them are validated by functional and expression studies. Even though trithorax proteins act by modulating chromatin structure at particular chromosomal locations, evidence of physical aggregation of ash2-regulated genes has not been found. This work represents the first microarray analysis, to our knowledge, of a trithorax-group gene.