Stimulation of ZPC Production by Follicle-Stimulating Hormone in the Granulosa Cells of Japanese Quail (Coturnix Japonica)
Stimulation of ZPC Production by Follicle-Stimulating Hormone in the Granulosa Cells of Japanese Quail (Coturnix Japonica)
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卵泡刺激素刺激日本鹌鹑 (Coturnix Japonica) 颗粒细胞中 ZPC 的产生
DOI：
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
M. Mori
中科院分区：
文献类型：
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作者：
Jianzhi Pan;T. Sasanami;M. Mori
禽卵黄周膜是一种与哺乳动物卵母细胞透明带同源的蛋白，由两种糖蛋白组成。先前的研究表明，其中一个成分，一种与哺乳动物ZPC同源的糖蛋白，是由鹌鹑卵巢成熟卵泡的颗粒细胞产生和分泌的。为了探讨ZPC产生的激素调节，在含有卵泡刺激素(FSH)、黄体生成素或催乳素的培养液中，比较了颗粒细胞[~3H]亮氨酸掺入ZPC的情况，并观察了FSH的刺激作用。通过Western blotting和Northern blotting分别检测培养液中的ZPC蛋白和细胞中ZPC的mRNA，我们发现加入FSH不仅能刺激颗粒细胞中ZPC蛋白的产生，而且还能刺激ZPC mRNA的表达。这些结果表明，FSH在基因转录水平上刺激ZPC蛋白的产生。
Avian perivitelline membrane, an investment homologous to the zona pellucida of mammalian oocytes, is composed of two glycoproteins. Previous studies have indicated that one of the components, a glycoprotein homologous to mammalian ZPC, is produced and secreted by the granulosa cells of maturing follicles in quail ovary. In the present study, to investigate the hormonal regulation of ZPC production, [3H] leucine incorporation into ZPC was compared in granulosa cells cultured in a medium containing follicle-stimulating hormone (FSH), luteinizing hormone, or prolactin, and a stimulatory effect of FSH was observed. By measuring ZPC protein in the medium and mRNA in the cells with Western blotting and Northern blotting analyses, respectively, we found that addition of FSH stimulated not only ZPC protein production but also ZPC mRNA expression in the granulosa cells. These results suggested that FSH stimulates ZPC protein production at the gene transcription level.
DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
兔卵泡中 55-kDa 透明带蛋白和信使 RNA 的发育表达。
影响因子：
2.7
作者：
Lee,VH;Dunbar,BS
通讯作者：
Dunbar,BS