Processing and antigenicity of entire and anchor-free spike glycoprotein S of coronavirus TGEV expressed by recombinant baculovirus.
Processing and antigenicity of entire and anchor-free spike glycoprotein S of coronavirus TGEV expressed by recombinant baculovirus.
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DOI：
10.1016/0042-6822(91)90544-l
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发表时间：
1991-12
期刊：
影响因子：
3.7
通讯作者：
Laude H
中科院分区：
文献类型：
--
作者：
Godet M;Rasschaert D;Laude H
用转移质粒pVL941将传染性胃肠炎病毒（TGEV）的S基因插入到加州签名虫核型多角体病毒（AcNPV）基因组中。重组病毒感染Sf9昆虫细胞后，合成了一个175K的多肽，该多肽能够三聚体化，并作为真正的TGEV S蛋白转运到细胞表面。尽管缺乏完整的碳水化合物加工，重组S蛋白表现出与TGEV S相似的抗原性，并在免疫大鼠中诱导高水平的中和抗体。在含有多肽膜锚点的羧基端进行基因工程缺失（70个氨基酸），使多肽得以分泌。结果表明，无锚蛋白的寡聚过程和抗原谱发生了部分改变。
The S gene of transmissible gastroenteritis virus (TGEV) was inserted into the genome of Autographa californica nuclear polyhedrosis virus (AcNPV) using the transfer plasmid pVL941. Infection of Sf9 insect cells with the recombinant virus resulted in the synthesis of a 175K polypeptide which was able to trimerize and was transported to the cell surface as is the authentic TGEV S protein. Despite the lack of complete carbohydrate processing, the recombinant S protein exhibited antigenic properties similar to TGEV S and induced high levels of neutralizing antibodies in immunized rats. Engineering a deletion (70 amino acids) into the carboxy-terminus containing the membrane anchor of the polypeptide allowed its secretion. The oligomerization process and the antigenic profile of the anchor-free S protein were shown to be partially altered.
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影响因子：
5.4
作者：
DOMS, RW;HELENIUS, A
通讯作者：
HELENIUS, A
DOI：
10.1016/0735-0651(88)90024-6
发表时间：
1988-03-01
期刊：
GENE ANALYSIS TECHNIQUES
影响因子：
--
作者：
LIM, HM;PENE, JJ
通讯作者：
PENE, JJ
影响因子：
56.9
作者：
SCHARF, SJ;HORN, GT;ERLICH, HA
通讯作者：
ERLICH, HA
影响因子：
5
作者：
Correa I;Jiménez G;Suñé C;Bullido MJ;Enjuanes L
通讯作者：
Enjuanes L
影响因子：
5.4
作者：
LITTELVANDENHURK, SV;PARKER, MD;BABIUK, LA
通讯作者：
BABIUK, LA