Molecular epidemiology and surveillance of circulating rotavirus among children with gastroenteritis in Bangladesh during 2014-2019.
Molecular epidemiology and surveillance of circulating rotavirus among children with gastroenteritis in Bangladesh during 2014-2019.
复制标题
DOI：
10.1371/journal.pone.0242813
复制
发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Ushijima H
中科院分区：
文献类型：
--
作者：
Dey SK;Sharif N;Sarkar OS;Sarkar MK;Talukder AA;Phan T;Ushijima H
急性胃肠炎是世界范围内5岁以下儿童的主要健康问题之一。轮状病毒A （RVA）是急性胃肠炎的重要病原体。在孟加拉国，小儿轮状病毒疾病的负担仍然很高。本研究调查了2014年2月至2019年1月孟加拉国急性胃肠炎儿童中A、B和C组轮状病毒（RAV、RBV、RCV）、诺如病毒、腺病毒（AdV）和人bocavavirus （HBoV）感染的流行情况。采用逆转录酶-多重聚合酶链反应（RT-多重PCR）对2014年2月至2019年1月期间从孟加拉国腹泻儿童中采集的574份粪便标本进行了RAV、RBV和RCV检测。采用rt -多重PCR和测序方法进一步鉴定RAV为g型和p型。574例粪便标本中RVA阳性140例（24.4%），AdV阳性4.5%；本研究未检测到RBV和RCV。基因型G1P[8]最常见（43%），其次是G2P[4]（18%）和G9P[8]（3%）。在其他基因型中，G9P[4]最常见（12%），其次是G1P[6] (11%), G9P[6]（3%）和G11P[25]（3%）。我们发现7%的RVA是不可分的。在G1P[8]和G2P[4]菌株中检测到VP7基因抗原区突变。11月至2月轮状病毒感染发生率最高（58.6%），以腹泻（90.7%）为最常见症状。4 ~ 11月龄儿童轮状病毒感染率最高（37.9%）。通过提供基线数据，本研究有助于评估目前可用的RVA疫苗的有效性。该研究揭示了高RVA检出率，支持卫生当局制定战略，如在国家免疫规划中引入RVA疫苗，以减轻疾病负担。
Acute gastroenteritis is one of the major health problems in children aged <5 years around the world. Rotavirus A (RVA) is an important pathogen of acute gastroenteritis. The burden of rotavirus disease in the pediatric population is still high in Bangladesh. This study investigated the prevalence of group A, B, and C rotavirus (RAV, RBV, RCV), norovirus, adenovirus (AdV) and human bocavirus (HBoV) infections in children with acute gastroenteritis in Bangladesh from February 2014 to January 2019. A total of 574 fecal specimens collected from children with diarrhea in Bangladesh during the period of February 2014-January 2019 were examined for RAV, RBV and RCV by reverse transcriptase- multiplex polymerase chain reaction (RT- multiplex PCR). RAV was further characterized to G-typing and P-typing by RT-multiplex PCR and sequencing method. It was found that 24.4% (140 of 574) fecal specimens were positive for RVA followed by AdV of 4.5%. RBV and RCV could not be detected in this study. Genotype G1P[8] was the most prevalent (43%), followed by G2P[4] (18%), and G9P[8] (3%). Among other genotypes, G9P[4] was most frequent (12%), followed by G1P[6] (11%), G9P[6] (3%), and G11P[25] (3%). We found that 7% RVA were nontypeable. Mutations at antigenic regions of the VP7 gene were detected in G1P[8] and G2P[4] strains. Incidence of rotavirus infection had the highest peak (58.6%) during November to February with diarrhea (90.7%) as the most common symptom. Children aged 4–11 months had the highest rotavirus infection percentage (37.9%). By providing baseline data, this study helps to assess efficacy of currently available RVA vaccine. This study revealed a high RVA detection rate, supporting health authorities in planning strategies such as introduction of RVA vaccine in national immunization program to reduce the disease burden.
登录
查看更多内容
影响因子：
14.9
作者：
Lefkowitz EJ;Dempsey DM;Hendrickson RC;Orton RJ;Siddell SG;Smith DB
通讯作者：
Smith DB
影响因子：
5.5
作者：
Lopez-Lacort, Monica;Collado, Susana;Diez-Domingo, Javier
通讯作者：
Diez-Domingo, Javier
影响因子：
5.5
作者：
Giri, Sidhartha;Hemavathy, R. Priya;Kang, Gagandeep
通讯作者：
Kang, Gagandeep
影响因子：
3.7
作者：
Jagai JS;Sarkar R;Castronovo D;Kattula D;McEntee J;Ward H;Kang G;Naumova EN
通讯作者：
Naumova EN
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro