Injectable in situ crosslinkable RGD-modified alginate matrix for endothelial cells delivery
Injectable in situ crosslinkable RGD-modified alginate matrix for endothelial cells delivery
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DOI：
10.1016/j.biomaterials.2011.07.013
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发表时间：
2011-11-01
期刊：
影响因子：
14
通讯作者：
Granja, Pedro L.
中科院分区：
文献类型：
--
作者：
Bidarra, Silvia J.;Barrias, Cristina C.;Granja, Pedro L.
以细胞为基础的治疗为组织的血管重建和再生提供了一种有吸引力的方法。然而，尽管临床迫切需要有效的血管重建策略，但成功地将活血管细胞固定在3D基质内是很难实现的。本文评估了天然可注射rgd -海藻酸盐水凝胶作为原位形成基质输送内皮细胞的体外潜力。研究人员采用了几种技术来研究这些微环境如何影响血管细胞的行为，即它们促进有活力的增殖细胞向外迁移的能力，并保持形成3D排列的能力。在rgd移植的海藻酸盐水凝胶中，细胞能够增殖，并在固定后至少48小时内保持80%的活力。此外，被包裹的细胞在细胞网络中创建了一个3D组织，当与基质接触时，细胞从rgd基质中迁移出来。总的来说，得到的结果支持了RGD肽结合海藻酸盐为内皮细胞的粘附、存活、迁移和组织提供了三维环境的观点。(C) 2011 Elsevier Ltd.版权所有。
Cell-based therapies offer an attractive approach for revascularization and regeneration of tissues. However, and despite the pressing clinical needs for effective revascularization strategies, the successful immobilization of viable vascular cells within 3D matrices has been difficult to achieve. In this paper the in vitro potential of a natural, injectable RGD-alginate hydrogel as an in situ forming matrix to deliver endothelial cells was evaluated. Several techniques were employed to investigate how these microenvironments could influence the behavior of vascular cells, namely their ability to promote the outward migration of viable, proliferative cells, retaining the ability to form a 3D arrangement. Cells within RGD-grafted alginate hydrogel were able to proliferate and maintained 80% of viability for at least 48 h post-immobilization. Additionally, entrapped cells created a 3D organization into cellular networks and, when put in contact with matrigel, cells migrated out of the RGD-matrix. Overall, the obtained results support the idea that the RGD peptides conjugated to alginate provide a 3D environment for endothelial cells adhesion, survival, migration and organization. (C) 2011 Elsevier Ltd. All rights reserved.