High-level synthesis of a heterologous milk protein in the mammary glands of transgenic swine.
High-level synthesis of a heterologous milk protein in the mammary glands of transgenic swine.
复制标题
转基因猪乳腺中异源乳蛋白的高水平合成。
DOI：
10.1073/pnas.88.5.1696
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发表时间：
1991
影响因子：
11.1
通讯作者：
Lothar Hennighausen
中科院分区：
文献类型：
--
作者：
R. Wall;V. Pursel;Avi Shamay;Robert A. Mcknight;C. Pittius;Lothar Hennighausen
乳清酸性蛋白（WAP）是小鼠、大鼠和兔子的主要乳蛋白，但尚未在包括猪在内的牲畜乳中发现。为了确定 WAP 基因的乳腺调节元件是否能够跨物种界限发挥作用，以及是否有可能定性地改变乳蛋白组成，我们将小鼠 WAP 基因引入猪的基因组中。对三个转基因猪系进行了分析，在所有哺乳期雌性的乳汁中均检测到小鼠 WAP，浓度约为 1 克/升；这些水平与小鼠奶中的水平相似。相应RNA的表达是乳腺特异性的。我们的结果表明，乳腺特异性基因表达的分子基础在猪和小鼠之间是保守的。此外，WAP 基因必须与其他乳蛋白基因共享将基因表达靶向乳腺的元件。小鼠WAP约占转基因猪乳汁总蛋白的3%，这表明在农场动物的乳汁中产生高水平的外源蛋白是可能的。可以产生高水平的外源蛋白质
The whey acidic protein (WAP) is a major milk protein in mice, rats, and rabbits but has not been found in milk of livestock including swine. To determine whether mammary gland regulatory elements from the WAP gene function across species boundaries and whether it is possible to qualitatively alter milk protein composition, we introduced the mouse WAP gene into the genome of swine. Three lines of transgenic swine were analyzed, and mouse WAP was detected in milk from all lactating females at concentrations of about 1 g/liter; these levels are similar to those found in mouse milk. Expression of the corresponding RNA was specific to the mammary gland. Our results suggest that the molecular basis of mammary-specific gene expression is conserved between swine and mouse. In addition the WAP gene must share, with other milk protein genes, elements that target gene expression to the mammary gland. Mouse WAP accounted for about 3% of the total milk proteins in transgenic pigs, thus demonstrating that it is possible to produce high levels of a foreign protein in milk of farm animals. that it is possible to produce high levels of a foreign protein in