A new assay for promoter analysis in Chlamydomonas reveals roles for heat shock elements and the TATA box in HSP70A promoter-mediated activation of transgene expression
A new assay for promoter analysis in Chlamydomonas reveals roles for heat shock elements and the TATA box in HSP70A promoter-mediated activation of transgene expression
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DOI：
10.1128/ec.00055-07
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发表时间：
2008-01-01
期刊：
影响因子：
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通讯作者：
Schroda, Michael
中科院分区：
文献类型：
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作者：
Lodha, Mukesh;Schulz-Raffelt, Miriam;Schroda, Michael
这项工作的目的是鉴定衣藻 HSP70A 启动子内的顺式调控序列，介导其对下游启动子表达的刺激作用。为此，我们删除/突变了 HSP70A 启动子，并使用新的测定法量化了其刺激效果。我们的结果表明，该效应主要是由热休克元件和 TATA 盒介导的。
The aim of this work was to identify cis-regulatory sequences within the Chlamydomonas HSP70A promoter that mediate its stimulatory effect on the expression of downstream promoters. For this, we deleted/mutated the HSP70A promoter and, using a new assay, quantified its stimulatory effect. Our results indicate that the effect is mediated largely by heat shock elements and the TATA box.