Wheat Oxophytodienoate Reductase Gene TaOPR1 Confers Salinity Tolerance via Enhancement of Abscisic Acid Signaling and Reactive Oxygen Species Scavenging
Wheat Oxophytodienoate Reductase Gene TaOPR1 Confers Salinity Tolerance via Enhancement of Abscisic Acid Signaling and Reactive Oxygen Species Scavenging
复制标题
小麦氧化植物二烯酸还原酶基因 TaOPR1 通过增强脱落酸信号传导和活性氧清除赋予耐盐性
DOI：
10.1104/pp.112.211854
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发表时间：
2013-03-01
期刊：
影响因子：
7.4
通讯作者：
Xia, Guangmin
中科院分区：
文献类型：
--
作者：
Dong, Wei;Wang, Mengcheng;Xia, Guangmin
12-氧代-植物二烯酸还原酶 (OPR) 分为两个亚组 OPRI 和 OPRII。后者蛋白质参与茉莉酸合成，而前者的功能尚不清楚。我们在此描述了 OPRI 基因 TaOPR1 的特征，该基因从耐盐面包小麦 (Triticum aestivum) 品种 SR3 中分离出来。盐度胁迫诱导 cv SR3 幼苗根部 TaOPR1 表达水平高于其亲本品种 JN177。当脱落酸（ABA）合成受到抑制时，这种诱导作用就会消失。 TaOPR1在小麦中的过表达显着增强了耐盐性水平，而其在拟南芥中的异源表达减轻了盐度和氧化剂存在下根系生长的限制，并提高了对ABA的敏感性。在拟南芥中，TaOPR1促进ABA合成和ABA依赖的胁迫响应途径，部分挽救了ABA合成缺陷的拟南芥aba2突变体对盐度的敏感性，提高活性氧清除剂的活性及其编码基因的转录，同时降低丙二醛和活性氧水平。 TaOPR1 不与茉莉酸合成或茉莉酸信号通路相互作用。我们相信 TaOPR1 并非纯粹作为抗氧化剂，而是在非生物应激反应期间作为与 ABA 介导的信号网络调节相关的信号化合物发挥作用。
The 12-oxo-phytodienoic acid reductases (OPRs) are classified into the two subgroups OPRI and OPRII. The latter proteins participate in jasmonic acid synthesis, while the function of the former ones is as yet unclear. We describe here the characterization of the OPRI gene TaOPR1, isolated from the salinity-tolerant bread wheat (Triticum aestivum) cultivar SR3. Salinity stress induced a higher level of TaOPR1 expression in the seedling roots of cv SR3 than in its parental cultivar, JN177. This induction was abolished when abscisic acid (ABA) synthesis was inhibited. The overexpression of TaOPR1 in wheat significantly enhanced the level of salinity tolerance, while its heterologous expression in Arabidopsis alleviated root growth restriction in the presence of salinity and oxidants and raised the sensitivity to ABA. In Arabidopsis, TaOPR1 promoted ABA synthesis and the ABA-dependent stress-responsive pathway, partially rescued the sensitivity of the Arabidopsis aba2 mutant defective in ABA synthesis to salinity, and improved the activities of reactive oxygen species scavengers and the transcription of their encoding genes while reducing malondialdehyde and reactive oxygen species levels. TaOPR1 did not interact with jasmonate synthesis or the jasmonate signaling pathway. Rather than serving purely as an antioxidant, we believe that TaOPR1 acts during episodes of abiotic stress response as a signaling compound associated with the regulation of the ABA-mediated signaling network.