Proteomic analysis of rice seedlings infected by Sinorhizobium meliloti 1021
Proteomic analysis of rice seedlings infected by Sinorhizobium meliloti 1021
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DOI：
10.1002/pmic.200900694
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发表时间：
2010-05-01
期刊：
影响因子：
3.4
通讯作者：
Shen, Shihua
中科院分区：
文献类型：
--
作者：
Chi, Feng;Yang, Pingfang;Shen, Shihua
根瘤内生菌不仅感染并定殖豆科植物，而且也感染并定殖若干非豆科物种。利用绿色荧光蛋白标记技术，已经证明根瘤菌感染不同品种的水稻物种，并从植物根部迁移到气生组织，如叶鞘和叶片。它们之间的相互作用促进了水稻的生长。生长促进是增强光合作用和抗逆性的累积结果。此外，吲哚-3-乙酸也有助于促进。为了解苜蓿中华根瘤菌1021对水稻根、叶鞘和叶片的影响机制，采用凝胶电泳比较蛋白质组学方法，对水稻根、叶鞘和叶片3种不同组织蛋白质组进行分析。接种根瘤菌后，参与9个不同功能类别的蛋白质被上调或下调。光合作用相关蛋白仅在叶鞘和叶片中表达上调，而根中表达上调的蛋白仅与防御相关。这一结果表明，光暗反应中的蛋白质向叶绿体的输入和运输增加，营养物质的分配效率提高，从而增强了光合作用。虽然防御反应的启动主要发生在根中，但在气生组织中也诱发了一些不同的防御机制。
Rhizobial endophytes infect and colonize not only leguminous plants, but several non-leguminous species as well. Using green fluorescent protein tagging technique, it has been shown that Rhizobia infect different varieties of rice species and migrate from plant roots to aerial tissues such as leaf sheaths and leaves. The interaction between them was found to promote the growth of rice. The growth promotion is the cumulative result of enhanced photosynthesis and stress resistance. In addition, indole-3-acetic acid also contributes to the promotion. Gel-based comparative proteomic approaches were applied to analyze the protein profiles of three different tissues (root, leaf sheath and leaf) of Sinorhizobium meliloti 1021 inoculated rice in order to get an understanding about the molecular mechanism. Upon the inoculation of rhizobia, proteins involved in nine different functional categories were either up-regulated or down-regulated. Photosynthesis related proteins were up-regulated only in leaf sheath and leaf, while the up-regulated proteins in root were exclusively defense related. The results implied that there might have been an increase in the import and transport of proteins involved in light and dark reactions to the chloroplast as well as more efficient distribution of nutrients, hence enhanced photosynthesis. Although the initiation of defensive reactions mainly occurred in roots, some different defense mechanisms were also evoked in the aerial tissues.