Human dendritic cells are superior to B cells at presenting a major histocompatibility complex class II-restricted heterologous antigen expressed on recombinant Streptococcus gordonii
Human dendritic cells are superior to B cells at presenting a major histocompatibility complex class II-restricted heterologous antigen expressed on recombinant Streptococcus gordonii
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DOI：
10.1128/iai.68.4.1879-1883.2000
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发表时间：
2000-04-01
影响因子：
3.1
通讯作者：
Girolomoni, G
中科院分区：
文献类型：
--
作者：
Corinti, S;Medaglini, D;Girolomoni, G
人们正在积极研究细菌作为疫苗载体来诱导或增强保护性免疫反应。在这项研究中，比较了人单核细胞来源的树突状细胞（DC）和正常B细胞将重组戈登链球菌表面表达的破伤风毒素（TTFC）C片段呈递给特异性CD4（+）T淋巴细胞的能力。 DC 在呈递可溶性 TTFC 方面比 B 细胞更有效，并且在获得给定 T 细胞反应所需的抗原量和每个细胞的基础上，更能够呈递细菌相关的 TTFC。这种差异与 B 细胞内吞可溶性 TTFC 和吞噬重组戈登沙门氏菌的能力低得多有关。此外，S. gordonii 诱导 DC 的表型成熟，但不诱导 B 细胞的表型成熟。因此，结果表明，DC而非B细胞在由重组革兰氏阳性菌免疫诱导的Ii类限制性免疫应答的放大中发挥着至关重要的作用。
Bacteria are being actively investigated as vaccine carriers for inducing or boosting protective immune responses. In this study, human monocyte-derived dendritic cells (DCs) and normal B cells were compared for their capacity to present the C fragment of tetanus toxin (TTFC), expressed on the surface of recombinant Streptococcus gordonii, to specific CD4(+) T lymphocytes. DCs were more efficient than B cells at presenting soluble TTFC and remarkably more capable of presenting bacterium-associated TTFC both in terms of the amount of antigen required to obtain a given T-cell response and on a per-cell basis. This difference was associated with a much lower capacity of B cells to endocytose soluble TTFC and phagocytose recombinant S. gordonii. In addition, S. gordonii induced the phenotypic maturation of DCs but not of B cells. The results thus indicate that DCs but not B cells play a crucial role in the amplification of class Ii-restricted immune responses induced by immunization with recombinant gram-positive bacteria.