Trichostatin A and nuclear reprogramming of cloned rabbit embryos
Trichostatin A and nuclear reprogramming of cloned rabbit embryos
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DOI：
10.2527/jas.2007-0718
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发表时间：
2008-05-01
影响因子：
3.3
通讯作者：
Chen, D. Y.
中科院分区：
文献类型：
--
作者：
Shi, L. H.;Ai, J. S.;Chen, D. Y.
为了研究组蛋白去乙酰化酶对核移植后核重编程的影响，我们用组蛋白去乙酰化酶抑制剂曲古抑菌素A（TSA）处理克隆胚胎。在本研究中，在组蛋白H3-赖氨酸14，组蛋白H4-赖氨酸12，组蛋白H4-赖氨酸5乙酰化的全球变化进行了研究，在兔体内受精胚胎，体细胞核移植（SCNT）胚胎，TSA处理的SCNT胚胎。从原核期到桑椹胚期，组蛋白H3-赖氨酸14和组蛋白H4-赖氨酸12乙酰化的去乙酰化-再乙酰化变化发生在受精胚胎和TSA-Key处理的克隆胚胎中;然而，未处理的克隆胚胎中的分布模式没有显示出这种变化。更有趣的是，在克隆胚胎中的组蛋白H4-赖氨酸12的乙酰化的信号被检测在内细胞团和滋养外胚层，而TSA处理的克隆胚胎显示出相同的染色模式，受精胚胎和染色仅限于内细胞团。TSA处理的SCNT胚胎的组蛋白乙酰化模式似乎与正常胚胎的组蛋白乙酰化模式更相似，表明TSA可以改善核移植后的核重编程。
To investigate the influence of histone deacetylases on nuclear reprogramming after nuclear transfer, we treated the cloned embryos with a histone deacetylase inhibitor, Trichostatin A (TSA). In the present study, global changes in acetylation of histone H3-lysine 14, histone H4-lysine 12, and histone H4-lysine 5 were studied in rabbit in vivo fertilized embryos, somatic cell nuclear transfer (SCNT) embryos, and TSA-treated SCNT embryos. From the pronuclear to the morula stage, the deacetylation-reacetylation changes in acetylation of histone H3-lysine 14 and histone H4-lysine 12 occurred in both fertilized embryos and TSA-Key treated cloned embryos; however, the distribution pattern in untreated cloned embryos failed to display such changes. More interesting, the signal of acetylation of histone H4-lysine 12 in cloned embryos was detected in both the inner cell mass and the trophectoderm, whereas TSA-treated cloned embryos showed the same staining pattern as fertilized embryos and the staining was limited to the inner cell mass. The histone acetylation pattern of TSA-treated SCNT embryos appeared to be more similar to that of normal embryos, indicating that TSA could improve nuclear reprogramming after nuclear transfer.