Remarkable homology among the internal repeats of erythroid and nonerythroid spectrin.
Remarkable homology among the internal repeats of erythroid and nonerythroid spectrin.
复制标题
红系血影蛋白和非红系血影蛋白的内部重复序列具有显着的同源性。
DOI：
10.1073/pnas.82.17.5671
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发表时间：
1985
影响因子：
11.1
通讯作者：
Barker,JE
中科院分区：
文献类型：
--
作者：
Birkenmeier,CS;Bodine,DM;Repasky,EA;Helfman,DM;Hughes,SH;Barker,JE
通过对鸡平滑肌cDNA文库的直接免疫筛选，鉴定了非红细胞α-血影蛋白的cDNA克隆。用鸡α-血影蛋白特异性抗血清筛选在表达质粒pUC 8和pUC 9中制备的文库。鸡和小鼠不同组织的poly（A）+ RNA的印迹显示，cDNA与8-腺苷酸酶mRNA杂交。该cDNA与鸡基因组DNA的Southern印迹上的单拷贝序列杂交。克隆的完整核酸序列具有单个1419碱基开放阅读框。衍生的氨基酸序列被组织成两个部分和三个完整的106-氨基酸重复序列，其显示出与针对人红细胞α-和β-血影蛋白所描述的重复序列的同源性。免疫学和生物化学数据表明，鸡非红细胞和人红细胞α-血影蛋白是血影蛋白家族中两个分歧较大的成员。在这方面，它们之间发现的同源性程度是出乎意料的。我们的数据表明，这两个α-血影蛋白的共同进化起源，并允许有关血影蛋白基因结构的一些预测。
A cDNA clone for nonerythroid alpha-spectrin was identified by direct immunological screening of a chicken smooth muscle cDNA library. A library prepared in the expression plasmids pUC8 and pUC9 was screened with an antiserum specific for chicken alpha-spectrin. Blots of poly(A)+ RNA from various tissues of chicken and mouse show that the cDNA hybridizes to an 8-kilobase mRNA. The cDNA hybridizes to a single-copy sequence on Southern blots of chicken genomic DNA. The complete nucleic acid sequence of the clone has a single 1419-base open reading frame. The derived amino acid sequence is organized into two partial and three complete 106-amino-acid repeats that show homology to the repeats described for human erythroid alpha- and beta-spectrin. Immunological and biochemical data indicate that chicken nonerythroid and human erythroid alpha-spectrin are two of the more widely diverged members of the spectrin family of proteins. In this respect, the degree of homology found between them was unexpected. Our data suggest a common evolutionary origin for these two alpha-spectrins and allow some predictions concerning spectrin gene structure.