meRanTK: methylated RNA analysis ToolKit
meRanTK: methylated RNA analysis ToolKit
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DOI：
10.1093/bioinformatics/btv647
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发表时间：
2016-03-01
期刊：
影响因子：
5.8
通讯作者：
Trajanoski, Zlatko
中科院分区：
文献类型：
--
作者：
Rieder, Dietmar;Amort, Thomas;Trajanoski, Zlatko
The significance and function of posttranscriptional cytosine methylation in poly(A) RNA attracts great interest but is still poorly understood. High-throughput sequencing of RNA treated with bisulfite (RNA-BSseq) or subjected to enrichment techniques like Aza-IP or miCLIP enables transcriptome wide studies of this particular modification at single base pair resolution. However, to date, there are no specialized software tools available for the analysis of RNA-BSseq or Aza-IP data. Therefore, we developed meRanTK, the first publicly available tool kit which addresses the special demands of high-throughput RNA cytosine methylation data analysis. It provides fast and easy to use splice-aware bisulfite sequencing read mapping, comprehensive methylation calling and identification of differentially methylated cytosines by statistical analysis of single-and multireplicate experiments. Application of meRanTK to RNA-BSseq or Aza-IP data produces accurate results in standard compliant formats.
