Analysis by High-Performance Liquid Chromatography Diode Array Detection Mass Spectrometry of Phenolic Compounds in Fruit of Eucalyptus globulus Cultivated in Algeria
Analysis by High-Performance Liquid Chromatography Diode Array Detection Mass Spectrometry of Phenolic Compounds in Fruit of Eucalyptus globulus Cultivated in Algeria
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DOI：
10.1021/jf1029509
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发表时间：
2010-12-22
影响因子：
6.1
通讯作者：
Madani, Khodir
中科院分区：
文献类型：
--
作者：
Boulekbache-Makhlouf, Lila;Meudec, Emmanuelle;Madani, Khodir
开发了一种基于高效液相色谱、二极管阵列检测和电喷雾电离质谱 (HPLC-DAD-ESI-MS) 并在 Sephadex LH-20 柱上进行色谱分离的方法，用于测定阿尔及利亚生长的蓝桉果实的酚类成分。已确定存在 18 种棓单宁、26 种鞣花单宁和 2 种黄酮醇。根据紫外-可见光谱和质谱数据对这些化合物进行了初步鉴定。本研究中描述的大多数化合物以前从未在蓝桉果实中检测到。此外，这是桉属中首次报道甲基二没食子酰二葡萄糖、3,3'-O-二甲基鞣花酸4-O-β-吡喃葡萄糖苷、鞣花酸己糖、甲基鞣花酸戊糖、甲基四没食子酰葡萄糖和缬酮酸异构体（山梨酸、黄酮没食子酸双内酯）。
A method based on high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) following fractionation by chromatography on a Sephadex LH-20 column has been developed to determine the phenolic composition of fruit of Eucalyptus globulus growing in Algeria. The presence of 18 gallotannins, 26 ellagitannins, and 2 flavonols was established. Tentative identification is provided for these compounds on the basis of UV-visible spectra and mass spectrometry data. Most compounds described in this study have not previously detected in fruit of E. globulus. Moreover, this is the first report of methyl digalloyl diglucose, 3,3'-O-dimethylellagic acid 4-O-beta-glucopyranoside, ellagic acid hexose, methyl ellagic acid pentose, methyltetragalloylglucose, and valoneic acid isomers (sanguisorbic, flavogallic acid dilactone) in the genus Eucalyptus.