Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
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DOI：
10.1515/bc.1999.045
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
Michael P. Rudolf;J. Nieland;Diane M. DaSilva;M. Velders;Martin Müller;Heather L. Greenstone;John T. Schiller;W. Kast
中科院分区：
文献类型：
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作者：
Michael P. Rudolf;J. Nieland;Diane M. DaSilva;M. Velders;Martin Müller;Heather L. Greenstone;John T. Schiller;W. Kast
Abstract It has been postulated that upon binding to a cell surface receptor, papilloma virus-like particles (VLPs) gain entry into the cytosol of infected cells and the capsid proteins L1 and L2 can be processed in the MHC class I presentation pathway. Vaccination of mice with human papilloma virus-like particles consisting of capsid proteins L1 and L2 induced a CD8- mediated and perforin dependent protective immune response against a tumor challenge with human papilloma virus transformed tumor cells, which express only minute amounts of L1 protein. Here we show that HPV16 capsid proteins stimulate a MHC class I restricted CTL response with human peripheral blood lymphocytes (PBL) in vitro. The vigorous response was specific for VLP-infected target cells and was MHC class I restricted. Moreover we show the presence of at least one HLA-A*0201 restricted CTL epitope within the HPV-16 capsid proteins by using a VLP-‘infected’ HLA-A*0201 transfected human cell line as target cells. These results demonstrated that VLPs can induce a HPV16 capsid protein-specific immune response in humans, allowing the monitoring of immune responses induced by vaccines based on chimeric VLPs carrying additional immunogenic peptides or proteins in therapeutical applications in human patients.
