Pro-Inflammatory Effects of Indoxyl Sulfate in Mice: Impairment of Intestinal Homeostasis and Immune Response.
Pro-Inflammatory Effects of Indoxyl Sulfate in Mice: Impairment of Intestinal Homeostasis and Immune Response.
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硫酸吲哚酚在小鼠中的促炎作用：损害肠道稳态和免疫反应。
DOI：
10.3390/ijms22031135
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发表时间：
2021-01-24
影响因子：
5.6
通讯作者：
Marzocco S
中科院分区：
文献类型：
--
作者：
Rapa SF;Prisco F;Popolo A;Iovane V;Autore G;Di Iorio BR;Dal Piaz F;Paciello O;Nishijima F;Marzocco S
The intestines are recognized as the main source of chronic inflammation in chronic kidney disease (CKD) and, among other cells, macrophages are involved in modulating this process as well as in the impaired immune response which also occurs in CKD patients. In this study, we evaluated the effect of Indoxyl Sulfate (IS), a protein bound uremic toxin poorly eliminated by hemodialysis, on inflammatory, oxidative stress and pro-apoptotic parameters, at the intestinal level in mice, on intestinal epithelial cells (IEC-6) and on primary murine peritoneal macrophages. C57BL/6J mice were treated with IS (800 mg/kg i.p.) for 3 or 6 h and histopathological analysis showed that IS induced intestinal inflammation and increased cyclooxygenase-2 (COX-2), nitrotyrosine and Bax expression in intestinal tissue. In IEC-6 cells, IS (125–1000 µM) increased tumor necrosis factor-α levels, COX-2 and inducible nitric oxide synthase expression and nitrotyrosine formation. Moreover, IS increased pro-oxidant, pro-inflammatory and pro-apoptotic parameters in peritoneal macrophages from IS-treated mice. Also, the serum concentration of IS and pro-inflammatory levels of cytokines resulted increased in IS-treated mice. Our results indicate that IS significantly contributes to affect intestinal homeostasis, immune response, and to induce a systemic pro-inflammatory state thus highlighting its potential role as therapeutic target in CKD patients.
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影响因子：
3.4
作者：
Han KH;Park JM;Jeong M;Han YM;Go EJ;Park J;Kim H;Han JG;Kwon O;Hahm KB
通讯作者：
Hahm KB
影响因子：
5.6
作者：
Adesso S;Magnus T;Cuzzocrea S;Campolo M;Rissiek B;Paciello O;Autore G;Pinto A;Marzocco S
通讯作者：
Marzocco S
影响因子：
5.6
作者：
Basilicata, Manuela Giovanna;Pepe, Giacomo;Campiglia, Pietro
通讯作者：
Campiglia, Pietro
DOI：
10.2215/cjn.02720409
发表时间：
2009-12-01
影响因子：
9.8
作者：
Carrero, Juan Jesus;Stenvinkel, Peter
通讯作者：
Stenvinkel, Peter
影响因子：
19.6
作者：
Contreras-Velazquez, J-C;Soto, V.;Paniagua, R.
通讯作者：
Paniagua, R.