IDENTIFICATION OF A CDNA FOR A HUMAN HIGH-MOLECULAR-WEIGHT B-CELL GROWTH-FACTOR
IDENTIFICATION OF A CDNA FOR A HUMAN HIGH-MOLECULAR-WEIGHT B-CELL GROWTH-FACTOR
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DOI：
10.1073/pnas.90.13.6330
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发表时间：
1993-07-01
影响因子：
11.1
通讯作者：
FORD, RJ
中科院分区：
文献类型：
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作者：
AMBRUS, JL;PIPPIN, J;FORD, RJ
增殖对于b细胞成熟过程中发生的许多表型变化是必要的。成熟B细胞进一步分化为浆细胞或记忆B细胞需要额外的增殖。在这篇文章中，我们描述了一个人类b细胞生长因子的cDNA，我们称之为高分子量b细胞生长因子（HMW-BCGF）。纯化的HMW-BCGF已被证明可以诱导b细胞增殖，抑制免疫球蛋白分泌，并选择性地扩增某些b细胞亚群。利用HMW-BCGF及其受体抗体的研究表明，HMW-BCGF虽然由T细胞和一些恶性B细胞产生，但主要作用于正常和恶性B细胞。通过HMW-BCGF单克隆抗体和多克隆抗血清的表达克隆，鉴定了HMW-BCGF cDNA。CDNA产生的蛋白诱导b细胞增殖，抑制免疫球蛋白分泌，并在免疫印迹中被抗hmw - bcgf抗体识别。从cDNA中推断出HMW-BCGF的氨基酸序列，预测其分泌蛋白为53 kDa，具有3个潜在的n -连锁糖基化位点。该cDNA的鉴定将有助于进一步研究该细胞因子的生理作用。我们建议将其命名为白细胞介素14。
Proliferation is necessary for many of the phenotypic changes that occur during B-cell maturation. Further differentiation of mature B cells into plasma cells or memory B cells requires additional rounds of proliferation. In this manuscript, we describe a cDNA for a human B-cell growth factor we call high-molecular-weight B-cell growth factor (HMW-BCGF). Purified HMW-BCGF has been shown to induce B-cell proliferation, inhibit immunoglobulin secretion, and selectively expand certain B-cell subpopulations. Studies using antibodies to HMW-BCGF and its receptor have suggested that HMW-BCGF, while produced by T cells and some malignant B cells, acts predominantly on normal and malignant B cells. The HMW-BCGF cDNA was identified by expression cloning using a monoclonal antibody and polyclonal antisera to HMW-BCGF. Protein produced from the CDNA induced B-cell proliferation, inhibited immunoglobulin secretion, and was recognized in immunoblots by anti-HMW-BCGF antibodies. The amino acid sequence of HMW-BCGF deduced from the cDNA predicts a secreted protein of 53 kDa with three potential N-linked glycosylation sites. The identification of this cDNA will allow further studies examining physiologic roles of this cytokine. We propose to call it interleukin 14.