Interleukin-18 regulates T helper 1 or 2 immune responses of human cord blood CD4+ V alpha 24+V beta 11+ natural killer T cells.
Interleukin-18 regulates T helper 1 or 2 immune responses of human cord blood CD4+ V alpha 24+V beta 11+ natural killer T cells.
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DOI：
10.3892/ijmm.20.2.241
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发表时间：
2007-08
影响因子：
5.4
通讯作者：
Yuka Fujibayashi;Y. Fujimori;Ikuyo Kasumoto;S. Kai;H. Hara;H. Okamura;H. Tsutsui;H. Ogawa;K. Nakanishi
中科院分区：
文献类型：
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作者：
Yuka Fujibayashi;Y. Fujimori;Ikuyo Kasumoto;S. Kai;H. Hara;H. Okamura;H. Tsutsui;H. Ogawa;K. Nakanishi
自然杀伤T(NKT)细胞同时具有T细胞和NK细胞标志，通过分泌T辅助细胞(Th)1和Th2细胞因子来调节免疫反应。我们分析了脐带血(CB)中NKT细胞的表型和功能特征以及控制Th1和Th2决定的调控机制。人CB Vα24+Vβ11+NKT细胞以CD4+单阳性(SP)表型为主(约96%)，而成人外周血Vα24+Vβ11+NKT细胞主要由CD4-CD8双阴性(DN)表型和少量CD4+SP表型组成。脐血CD4+Vα24+NKT细胞经原代培养后，获得了分泌Th1细胞因子干扰素-γ和Th2细胞因子IL-13的能力。IL-18和IL-12联合作用可诱导脐血CD4+Vα24+NKT细胞产生干扰素-γ，而IL-18和IL-2联合作用可诱导这些细胞产生IL-13。因此，IL-18通过不同的细胞因子组合调节人类CD4+Vα24+NKT细胞对Th1或Th2免疫应答的决定。
Natural killer T (NKT) cells, exhibiting both T-cell and NK-cell markers, are known to regulate immune responses by secreting T-helper (Th) 1 and Th2 cytokines. We analyzed NKT cells in cord blood (CB) for phenotypical and functional characteristics and regulatory mechanisms that control Th1 and Th2 determination. Human CB V alpha 24+V beta 11+ NKT cells were predominantly the CD4+ single positive (SP) phenotype (approximately 96%), in contrast to adult peripheral blood V alpha 24+V beta 11+ NKT cells which are composed of a dominant population of the CD4-CD8-double negative (DN) phenotype and a minor population of the CD4+ SP phenotype. The CB CD4+ V alpha 24+ NKT cells, following stimulation with the primary culture, gained the capacity to secrete interferon (IFN)-gamma, a Th1 cytokine, and interleukin (IL)-13, a Th2 cytokine. The combination of IL-18 and IL-12 induced IFN-gamma production in CB CD4+ V alpha 24+ NKT cells, while IL-18 in combination with IL-2 induced IL-13 production in these cells. Thus, IL-18 regulates the determination of the Th1 or Th2 immune response by human CD4+ V alpha 24+ NKT cells through different cytokine combinations.