A single residue mutation of 5-enoylpyruvylshikimate-3-phosphate synthase in Pseudomonas stutzeri enhances resistance to the herbicide glyphosate
A single residue mutation of 5-enoylpyruvylshikimate-3-phosphate synthase in Pseudomonas stutzeri enhances resistance to the herbicide glyphosate
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DOI：
10.1007/s10529-008-9703-8
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发表时间：
2008-08-01
影响因子：
2.7
通讯作者：
Lin, Min
中科院分区：
文献类型：
--
作者：
Liang, Aimin;Sha, Jiying;Lin, Min
通过互补大肠杆菌aroA营养缺陷型突变体，从施氏假单胞菌A1501中鉴定出一种新的Ⅱ类5-烯酰异戊酰莽草酸-3-磷酸合酶（EPSPS）。A1501 EPSPS的丝氨酸（Ser）取代天冬酰胺（Asn）-130的单个氨基酸增强了对200 mM草甘膦的抗性。突变的EPSPS的IC 50 [草甘膦]值增加了2.5倍，Ki [草甘膦]值增加了2倍，但K-m [PEP]值与野生型相似。利用计算机三维模型分析了单残基突变对草甘膦抗性的影响。
A novel class II 5-enoylpyruvylshikimate-3-phosphate synthase (EPSPS) was identified from Pseudomonas stutzeri A1501 by complementation of an Escherichia coli auxotrophic aroA mutant. The single amino acid substitution of serine (Ser) for asparagine (Asn)-130 of the A1501 EPSPS enhanced resistance to 200 mM glyphosate. The mutated EPSPS had a 2.5-fold increase for IC50 [glyphosate] value, a 2-fold increase for K-i [glyphosate] value, but a K-m [PEP] value similar to that of wild type. The effect of the single residue mutation on glyphosate resistance was also analyzed using a computer-based three-dimensional model.