The role of matrix proteins in the control of nacreous layer deposition during pearl formation
The role of matrix proteins in the control of nacreous layer deposition during pearl formation
复制标题
基质蛋白在珍珠形成过程中控制珍珠层沉积的作用
DOI：
10.1098/rspb.2011.1661
复制
发表时间：
2012-03-07
影响因子：
4.7
通讯作者：
Zhang, Rongqing
中科院分区：
文献类型：
--
作者：
Liu, Xiaojun;Li, Jiale;Zhang, Rongqing
为研究珍珠母贝基质蛋白在珍珠层生物矿化中的作用，本研究检测了珍珠形成早期珍珠核的沉积和珍珠囊基质蛋白基因的表达。我们发现珍珠的形成过程包括两个连续的阶段：（i）不规则的碳酸钙（CaCO 3）沉积在裸核上，（ii）CaCO 3沉积变得越来越规则，直到成熟的珍珠层已经形成在核上。在珍珠囊中的基质蛋白的低表达水平期间不规则的碳酸钙沉积表明，沉积可能不受控制的有机基质在这个阶段的过程中。然而，在植入后30-35天检测到珍珠囊中基质蛋白的显著表达。在第30天，我们认为是无定形CaCO 3的CaCO 3薄层覆盖了大文石。到第35天，形成了珍珠层。整个过程与贝壳中观察到的相似，并且基质蛋白基因的时间表达表明其生物活性对珍珠发育至关重要。基质蛋白控制碳酸钙晶体的晶相、形状、大小、成核和聚集。
To study the function of pearl oyster matrix proteins in nacreous layer biomineralization in vivo, we examined the deposition on pearl nuclei and the expression of matrix protein genes in the pearl sac during the early stage of pearl formation. We found that the process of pearl formation involves two consecutive stages: (i) irregular calcium carbonate (CaCO3) deposition on the bare nucleus and (ii) CaCO3 deposition that becomes more and more regular until the mature nacreous layer has formed on the nucleus. The low-expression level of matrix proteins in the pearl sac during periods of irregular CaCO3 deposition suggests that deposition may not be controlled by the organic matrix during this stage of the process. However, significant expression of matrix proteins in the pearl sac was detected by day 30–35 after implantation. On day 30, a thin layer of CaCO3, which we believe was amorphous CaCO3, covered large aragonites. By day 35, the nacreous layer had formed. The whole process is similar to that observed in shells, and the temporal expression of matrix protein genes indicated that their bioactivities were crucial for pearl development. Matrix proteins controlled the crystal phase, shape, size, nucleation and aggregation of CaCO3 crystals.