Effect of inhibitory compounds found in biomass hydrolysates on growth and xylose fermentation by a genetically engineered strain of S-cerevisiae
Effect of inhibitory compounds found in biomass hydrolysates on growth and xylose fermentation by a genetically engineered strain of S-cerevisiae
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DOI：
10.1016/s0141-0229(03)00214-x
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发表时间：
2003-11-05
影响因子：
3.4
通讯作者：
Duff, S
中科院分区：
文献类型：
--
作者：
Helle, S;Cameron, D;Duff, S
将抑制剂对重组木糖发酵菌株酿酒酵母259ST的影响与三种参考菌株进行了比较，包括亲本菌株（S.酿酒酵母259A）和适应亚硫酸盐废液（SSL）的工业菌株。抑制剂之间的相互作用效应通过部分析因设计进行了验证。S.发现酿酒酵母259 ST对乙酸、铵、糠醛和渗透作用（其为SSL中的抑制性化合物）的耐受性与参考菌株一样哈代。铵毒性似乎是由于渗透效应。对于所有测试的抑制剂，葡萄糖发酵过程中，生长速率比乙醇产量受到更严重的抑制。S.酿酒酵母259ST发酵木糖的能力比发酵葡萄糖的能力受到更严重的影响。在软木SSL（pH 5）中预期的浓度下，乙酸将使生长速率降低15%，而木糖上的乙醇产率将降低50%，而铵将使酵母生长速率降低20%，并使木糖的乙醇产率降低45%。(C)2003年爱思唯尔公司All rights reserved.
The effect of inhibitors on the recombinant xylose fermenting strain Saccharomyces cerevisiae 259ST was compared to three reference strains, including the parent strain (S. cerevisiae 259A) and an industrial strain adapted to spent sulfite liquor (SSL). Interaction effects between the inhibitors were verified by a fractional factorial design. S. cerevisiae 259ST was found to be as hardy as the reference strains towards acetic acid, ammonium, furfural, and osmotic effects, which are inhibitory compounds in SSL. Ammonium toxicity appeared to be due to osmotic effects. For all of the inhibitors tested., growth rate was more severely inhibited than the ethanol yield during fermentation of glucose. The ability of S. cerevisiae 259ST to ferment xylose was more severely affected than the ability to ferment glucose. At the concentrations expected in softwood SSL (pH 5), acetic acid will decrease the growth rate by 15%, while the ethanol yield on xylose would decrease by 50%, while ammonium will decrease the yeast growth rate by 20% and decrease the ethanol yield from xylose by 45%. (C) 2003 Elsevier Inc. All rights reserved.