FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis
FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis
复制标题
FBXO47 通过稳定减数分裂过程中的 TRF2 来调节端粒-内核膜整合
DOI：
10.1093/nar/gkz992
复制
发表时间：
2019-12-16
影响因子：
14.9
通讯作者：
Liu, Mingxi
中科院分区：
文献类型：
--
作者：
Hua, Rong;Wei, Huafang;Liu, Mingxi
在减数分裂过程中，端粒附着在内核膜上是同源染色体正确配对和重组所必需的。在这里，我们确定了F-box蛋白47（FBXO 47）作为一个调节器的端粒shelterin复合物，在减数分裂前期I的具体表达。敲除小鼠中的Fbxo 47导致雄性不育。我们发现Fbxo 47缺陷的精母细胞不能形成完整的联会复合体。FBXO 47与TRF 1/2相互作用，Fbxo 47的破坏使TRF 2不稳定，导致不稳定的端粒附着和缓慢穿过花束阶段。我们的研究结果揭示了FBXO 47在减数分裂过程中稳定端粒shelterin亚基的新机制。
During meiosis, telomere attachment to the inner nuclear envelope is required for proper pairing of homologous chromosomes and recombination. Here, we identified F-box protein 47 (FBXO47) as a regulator of the telomeric shelterin complex that is specifically expressed during meiotic prophase I. Knockout of Fbxo47 in mice leads to infertility in males. We found that the Fbxo47 deficient spermatocytes are unable to form a complete synaptonemal complex. FBXO47 interacts with TRF1/2, and the disruption of Fbxo47 destabilizes TRF2, leading to unstable telomere attachment and slow traversing through the bouquet stage. Our findings uncover a novel mechanism of FBXO47 in telomeric shelterin subunit stabilization during meiosis.