Vesicular Glutamate and GABA Transporters Sort to Distinct Sets of Vesicles in a Population of Presynaptic Terminals
Vesicular Glutamate and GABA Transporters Sort to Distinct Sets of Vesicles in a Population of Presynaptic Terminals
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DOI：
10.1093/cercor/bhn077
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发表时间：
2009-01-01
期刊：
影响因子：
3.7
通讯作者：
Chaudhry, Farrukh A.
中科院分区：
文献类型：
--
作者：
Boulland, Jean-Luc;Jenstad, Monica;Chaudhry, Farrukh A.
囊泡谷氨酸转运蛋白（VGLUT）1和2由神经元表达，通常被接受释放谷氨酸作为神经递质，而VGLUT 3出现在通常与不同的经典递质相关的人群中。我们现在证明VGLUT2以及囊泡GABA转运体（VGAT）在大鼠海马结构的齿状回突触前末梢的子集。终末分布在与颗粒细胞层的外部和分子层的内部区域重叠的特征带中。在终端，这使得不对称以及对称的突触到齿状颗粒细胞的体树突隔室，2转运定位到不同的群体的突触囊泡。此外，形成这些终端的轴突起源于乳头体上核（SuM）。我们的数据调和以前显然是相互矛盾的报告，从南极洲的齿状传入的生理和证明，谷氨酸和GABA可能会从一个单一的神经末梢释放。
Vesicular glutamate transporters (VGLUTs) 1 and 2 are expressed by neurons generally accepted to release glutamate as a neurotransmitter, whereas VGLUT3 appears in populations usually associated with a different classical transmitter. We now demonstrate VGLUT2 as well as the vesicular GABA transporter (VGAT) in a subset of presynaptic terminals in the dentate gyrus of the rat hippocampal formation. The terminals are distributed in a characteristic band overlapping with the outer part of the granule cell layer and the inner zone of the molecular layer. Within the terminals, which make asymmetric as well as symmetric synapses onto the somatodendritic compartment of the dentate granule cells, the 2 transporters localize to distinct populations of synaptic vesicles. Moreover, the axons forming these terminals originate in the supramammillary nucleus (SuM). Our data reconcile previous apparently conflicting reports on the physiology of the dentate afferents from SuM and demonstrate that both glutamate and GABA may be released from a single nerve terminal.