Molecular Mechanisms and Pathways Involved in Bovine Embryonic Genome Activation and Their Regulation by Alternative In Vivo and In Vitro Culture Conditions
Molecular Mechanisms and Pathways Involved in Bovine Embryonic Genome Activation and Their Regulation by Alternative In Vivo and In Vitro Culture Conditions
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DOI：
10.1095/biolreprod.112.099697
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发表时间：
2012-10-01
影响因子：
3.6
通讯作者：
Tesfaye, Dawit
中科院分区：
文献类型：
--
作者：
Gad, Ahmed;Hoelker, Michael;Tesfaye, Dawit
了解植入前不同时间点环境条件变化的基因表达模式将提高我们对胚胎发育调控的认识。在这里，我们的目的是检查主要胚胎基因组激活（EGA）时的替代体内和体外培养条件对牛囊胚发育和转录组谱的影响。在主要 EGA 之前或之后，在不同的体内和体外培养条件下产生了四个不同的囊胚组。完全在体外和体内产生的囊胚用作对照。我们使用 EmbryoGENE 的牛微阵列比较了每个囊胚组和体内囊胚对照组之间的基因表达模式。数据表明，在主要 EGA 之前或之后，将培养条件从体内改为体外，反之亦然，对发育速率没有影响；然而，那段时间的体外条件严重影响了所产生的囊胚的转录组。卵母细胞的来源对发育速率和胚胎对不断变化的培养条件做出反应的能力具有至关重要的影响。本体论分类强调了体内和体外产生的囊胚之间脂质代谢和氧化应激反应的表达模式存在显着差异，且趋势相反。定义了 EGA 期间培养条件改变影响的分子机制和途径。这些结果将有助于制定新策略，在这个关键阶段改变培养条件，以促进有能力的囊胚的发育。
Understanding gene expression patterns in response to altered environmental conditions at different time points of the preimplantation period would improve our knowledge on regulation of embryonic development. Here we aimed to examine the effect of alternative in vivo and in vitro culture conditions at the time of major embryonic genome activation (EGA) on the development and transcriptome profile of bovine blastocysts. Four different blastocyst groups were produced under alternative in vivo and in vitro culture conditions before or after major EGA. Completely in vitro-and in vivo-produced blastocysts were used as controls. We compared gene expression patterns between each blastocyst group and in vivo blastocyst control group using EmbryoGENE's bovine microarray. The data showed that changing culture conditions from in vivo to in vitro or vice versa, either before or after the time of major EGA, had no effect on the developmental rates; however, in vitro conditions during that time critically influenced the transcriptome of the blastocysts produced. The source of oocyte had a critical effect on developmental rates and the ability of the embryo to react to changing culture conditions. Ontological classification highlighted a marked contrast in expression patterns for lipid metabolism and oxidative stress response between blastocysts generated in vivo versus in vitro, with opposite trends. Molecular mechanisms and pathways that are influenced by altered culture conditions during EGA were defined. These results will help in the development of new strategies to modify culture conditions at this critical stage to enhance the development of competent blastocysts.