Adipophilin is a sensitive marker for lipid loading in human blood monocytes
Adipophilin is a sensitive marker for lipid loading in human blood monocytes
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DOI：
10.1016/s1388-1981(01)00121-4
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发表时间：
2001-05-31
影响因子：
4.8
通讯作者：
Schmitz, G
中科院分区：
文献类型：
--
作者：
Buechler, C;Ritter, M;Schmitz, G
亲脂蛋白是一种最初在脂肪细胞中描述的脂质积聚的标志物，最近显示在巨噬细胞中被诱导。我们发现，即使是新鲜分离的血液单核细胞表达亲脂蛋白，亲脂蛋白的量是可变的单核细胞从不同的健康个体。然而，亲脂蛋白的生理表达与游离脂肪酸、胆固醇酯或游离胆固醇的水平无关。酶修饰的低密度脂蛋白（E-LDL）诱导单核细胞中快速泡沫细胞形成，并在孵育2小时内上调adipophilin mRNA和蛋白质。这种亲脂蛋白的快速诱导伴随着与E-LDL孵育的单核细胞中游离脂肪酸的显著增加。Adipophilin促进游离脂肪酸的摄取，在这里，我们证明了游离脂肪酸的增加与血液单核细胞中adipophilin表达的早期上调有关。脂肪酸是过氧化物酶体增殖物激活受体-γ（PPARgamma）的配体，PPARgamma激动剂如15 d-PGJ（2）和ciglitazone可上调adipophilin mRNA，表明PPARgamma可介导人血单核细胞中adipophilin表达的诱导。(C)2001爱思唯尔科技有限公司。保留所有权利。
Adipophilin, a marker of lipid accumulation initially described in adipocytes, was recently shown to be induced in macrophage roam cells. We found that even freshly isolated blood monocytes express adipophilin and that the amount of adipophilin protein is variable in monocytes from different healthy individuals. However, the physiological expression of adipophilin does not correlate with the levels of free fatty acids, cholesterylesters or free cholesterol. Enzymatically modified low-density lipoprotein (E-LDL) induces rapid foam cell formation in monocytes and upregulates adipophilin mRNA and protein within 2 h of incubation. This rapid induction of adipophilin is accompanied by a significant increase of free fatty acids in monocytes incubated with E-LDL. Adipophilin facilitates the uptake of free fatty acids, and here we demonstrate that free fatty acids increase is related to the early upregulation of adipophilin expression in blood monocytes. Fatty acids are ligands for peroxisome proliferator-activated receptor-gamma (PPAR gamma), and the upregulation of adipophilin mRNA by PPAR gamma agonists like 15d-PGJ(2) and ciglitazone indicates that PPAR gamma may mediate the induction of adipophilin expression in human blood monocytes. (C) 2001 Elsevier Science B.V. All rights reserved.