Nonviral approaches for neuronal delivery of nucleic acids.
Nonviral approaches for neuronal delivery of nucleic acids.
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DOI：
10.1007/s11095-007-9439-5
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发表时间：
2008-05
影响因子：
3.7
通讯作者：
Pun, Suzie H.
中科院分区：
文献类型：
--
作者：
Bergen, Jamie M.;Park, In-Kyu;Horner, Philip J.;Pun, Suzie H.
将治疗性核酸输送到神经元具有治疗神经系统疾病和脊髓损伤的潜力。虽然选择的病毒载体已经显示出作为神经元基因载体的希望，但它们作为治疗药物的潜力受到其毒性和免疫原性、广泛的亲和性和大规模配方成本的限制。非病毒载体是一种有吸引力的替代方案，因为与病毒相比，它们提供了更好的安全性，生产成本更低，并且可以针对特定的神经元亚群。然而，大多数非病毒载体的转染效率明显低于嗜神经病毒，严重限制了它们在神经元靶向传递应用中的应用。为了实现神经元中非病毒传递技术的潜力，必须设计载体以克服一系列细胞外和细胞内屏障。在这篇文章中，我们描述了阻止核酸成功非病毒传递到神经元的挑战，并回顾了旨在克服这些挑战的策略。
The delivery of therapeutic nucleic acids to neurons has the potential to treat neurological disease and spinal cord injury. While select viral vectors have shown promise as gene carriers to neurons, their potential as therapeutic agents is limited by their toxicity and immunogenicity, their broad tropism, and the cost of large-scale formulation. Nonviral vectors are an attractive alternative in that they offer improved safety profiles compared to viruses, are less expensive to produce, and can be targeted to specific neuronal subpopulations. However, most nonviral vectors suffer from significantly lower transfection efficiencies than neurotropic viruses, severely limiting their utility in neuron-targeted delivery applications. To realize the potential of nonviral delivery technology in neurons, vectors must be designed to overcome a series of extra- and intracellular barriers. In this article, we describe the challenges preventing successful nonviral delivery of nucleic acids to neurons and review strategies aimed at overcoming these challenges.
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影响因子：
--
作者：
Chiu, YL;Ali, A;Rana, TM
通讯作者：
Rana, TM
影响因子：
4.9
作者：
Bergen, Jamie M.;Pun, Suzie H.
通讯作者：
Pun, Suzie H.
DOI：
10.1007/bf03206650
发表时间：
2005-01-01
期刊：
NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics
影响因子：
--
作者：
Boado, Ruben J
通讯作者：
Boado, Ruben J
影响因子：
5.3
作者：
Barati, S;Chegini, F;Rush, RA
通讯作者：
Rush, RA
影响因子：
5.3
作者：
Barati, Shahram;Hurtado, Plinio R.;Rush, Robert A.
通讯作者：
Rush, Robert A.