Diversity of ganglion cells in the mouse retina: Unsupervised morphological classification and its limits
Diversity of ganglion cells in the mouse retina: Unsupervised morphological classification and its limits
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DOI：
10.1002/cne.20631
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发表时间：
2005-08-29
影响因子：
2.5
通讯作者：
Masland, RH
中科院分区：
文献类型：
--
作者：
Kong, JH;Fish, DR;Masland, RH
通过颗粒介导的DiI转移、微量注射路西法黄或转基因菌株中表达的绿色荧光蛋白，可见小鼠视网膜神经节细胞的树突状结构。对219个细胞进行三维成像，定量分析细胞形态。共研究了26个参数，并采用k-means方法进行自动聚类分析。通过剪影分析判断，通过三个参数：分层水平、树突场的范围和分支密度来实现有效的聚类。给出了一个11个集群的解决方案。除了统计上用来形成集群的细胞外，每个集群内的细胞沿着形态维度明显相似。它们通常可以与以前研究中定义的神经节细胞类型相匹配。然而，由于讨论的原因，这种分类必须是临时的。指出了实现更明确的无监督分类方法的一些步骤。(c) 2005 Wiley-Liss, Inc。
The dendritic structures of retinal ganglion cells in the mouse retina were visualized by particle-mediated transfer of DiI, microinjection of Lucifer yellow, or visualization of green fluorescent protein expressed in a transgenic strain. The cells were imaged in three dimensions and the morphologies of a series of 219 cells were analyzed quantitatively. A total of 26 parameters were studied and automated cluster analysis was carried out using the k-means methods. An effective clustering, judged by silhouette analysis, was achieved using three parameters: level of stratification, extent of the dendritic field, and density of branching. An 11-cluster solution is illustrated. The cells within each cluster are visibly similar along morphological dimensions other than those used statistically to form the clusters. They could often be matched to ganglion cell types defined by previous studies. For reasons that are discussed, however, this classification must remain provisional. Some steps toward more definitive methods of unsupervised classification are pointed out. (c) 2005 Wiley-Liss, Inc.