The structure of DNA in the nucleosome core
The structure of DNA in the nucleosome core
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DOI：
10.1038/nature01595
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发表时间：
2003-05-08
期刊：
影响因子：
64.8
通讯作者：
Davey, CA
中科院分区：
文献类型：
--
作者：
Richmond, TJ;Davey, CA
含有 147 个 DNA 碱基对的核小体核心颗粒的 1.9 埃分辨率晶体结构以前所未有的精度揭示了核小体 DNA 的构象。 DNA 结构与寡核苷酸和非组蛋白蛋白质-DNA 复合物的结构显着不同。总体而言，DNA 碱基对阶梯几何形状的曲率是容纳核小体中 DNA 超螺旋路径所需的曲率的两倍。弯曲到小沟中的 DNA 片段要么扭结，要么交替移动。组蛋白结合诱导的不寻常 DNA 构象参数对序列依赖性蛋白质识别以及核小体定位和移动性具有影响。 147 个碱基对结构与两个 146 个碱基对结构的比较揭示了 DNA 扭曲的改变，这种改变在大量染色质中显然很常见，并且对于染色质纤维形成和染色质重塑可能很重要。
The 1.9-Angstrom-resolution crystal structure of the nucleosome core particle containing 147 DNA base pairs reveals the conformation of nucleosomal DNA with unprecedented accuracy. The DNA structure is remarkably different from that in oligonucleotides and nonhistone protein-DNA complexes. The DNA base-pair-step geometry has, overall, twice the curvature necessary to accommodate the DNA superhelical path in the nucleosome. DNA segments bent into the minor groove are either kinked or alternately shifted. The unusual DNA conformational parameters induced by the binding of histone protein have implications for sequence-dependent protein recognition and nucleosome positioning and mobility. Comparison of the 147-base-pair structure with two 146-base-pair structures reveals alterations in DNA twist that are evidently common in bulk chromatin, and which are of probable importance for chromatin fibre formation and chromatin remodelling.