Epstein-Barr virus BZLF1 gene, a switch from latency to lytic infection, is expressed as an immediate-early gene after primary infection of B lymphocytes
Epstein-Barr virus BZLF1 gene, a switch from latency to lytic infection, is expressed as an immediate-early gene after primary infection of B lymphocytes
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DOI：
10.1128/jvi.01416-06
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发表时间：
2007-01-01
影响因子：
5.4
通讯作者：
Takada, Kenzo
中科院分区：
文献类型：
--
作者：
Wen, Wangrong;Iwakiri, Dal;Takada, Kenzo
我们在这里证明，EB病毒（EBV）BZLF1基因，从潜伏感染到裂解感染的开关，早在1.5小时后，EBV感染伯基特淋巴瘤衍生的，EBV阴性的Akata和Daudi细胞和原代B淋巴细胞的表达。由于BZLF 1 mRNA即使在存在蛋白质合成抑制剂茴香霉素的情况下被EBV感染时也表达，因此其表达不需要先决的蛋白质合成，这表明BZLF 1在B淋巴细胞的原发性EBV感染后作为立即早期基因表达。
We demonstrate here that the Epstein-Barr virus (EBV) BZLF1 gene, a switch from latent infection to lytic infection, is expressed as early as 1.5 h after EBV infection in Burkitt's lymphoma-derived, EBV-negative Akata and Daudi cells and primary B lymphocytes. Since BZLF1 mRNA is expressed even when the cells are infected with EBV in the presence of anisomycin, an inhibitor of protein synthesis, its expression does not require prerequisite protein synthesis, indicating that BZLF1 is expressed as an immediate-early gene following primary EBV infection of B lymphocytes.