TRANSFORMING GROWTH-FACTOR TYPE-BETA SPECIFICALLY STIMULATES SYNTHESIS OF PROTEOGLYCAN IN HUMAN ADULT ARTERIAL SMOOTH-MUSCLE CELLS
TRANSFORMING GROWTH-FACTOR TYPE-BETA SPECIFICALLY STIMULATES SYNTHESIS OF PROTEOGLYCAN IN HUMAN ADULT ARTERIAL SMOOTH-MUSCLE CELLS
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DOI：
10.1073/pnas.84.15.5287
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发表时间：
1987-08-01
影响因子：
11.1
通讯作者：
MCKEEHAN, WL
中科院分区：
文献类型：
--
作者：
CHEN, JK;HOSHI, H;MCKEEHAN, WL
肌内膜蛋白聚糖代谢被认为在血管稳态、血液凝固、动脉粥样硬化形成和动脉粥样硬化中是重要的。人血小板衍生的转化生长因子β型（TGF-β）在培养的非增殖人成人动脉平滑肌细胞中特异性刺激至少两种类型的硫酸软骨素蛋白聚糖的合成。平滑肌细胞生长促进剂（表皮生长因子、血小板衍生生长因子和肝素结合生长因子）对平滑肌细胞蛋白聚糖合成的刺激小于TGF-β引起的刺激的20%。TGF-.beta.既不显著刺激静止平滑肌细胞的增殖，也不抑制增殖细胞。TGF-β的程度平滑肌细胞蛋白聚糖合成的刺激在非增殖和生长刺激的细胞中是相似的。TGF-β，其是内皮细胞增殖的可逆抑制剂，对内皮细胞蛋白聚糖合成没有类似的作用。这些结果与TGF-β抑制剂抑制肿瘤生长的假设一致。是人血管中蛋白聚糖合成的细胞类型特异性调节剂，可能有助于动脉粥样硬化病变中蛋白聚糖的肌内膜积聚。
Myo-intimal proteoglycan metabolism is thought to be important in blood vessel homeostasis, blood clotting, atherogenesis, and atherosclerosis. Human platelet-derived transforming growth factor type .beta. (TGF-.beta.) specifically stimulated synthesis of at least two types of chondroitin sulfate proteoglycans in nonproliferating human adult arterial smooth muscle cells in culture. Stimulation of smooth muscle cell proteoglycan synthesis by smooth muscle cell growth promoters (epidermal growth factor, platelet-derived growth factor, and heparin-binding growth factors) was < 20% of that elicited by TGF-.beta.. TGF-.beta. neither significantly stimulated proliferation of quiescent smooth muscle cells nor inhibited proliferating cells. The extent of TGF-.beta. stimulation of smooth muscle cell proteoglycan synthesis was similar in both non-proliferating and growth-stimulated cells. TGF-.beta., which is a reversible inhibitor of endothelial cell proliferation, had no comparable effect on endothelial cell proteoglycan synthesis. These results are consistent with the hypothesis that TGF-.beta. is a cell-type-specific regulator of proteoglycan synthesis in human blood vessels and may contribute to the myo-intimal accumulation of proteoglycan in atherosclerotic lesions.