Differential receptors create patterns diagnostic for ATP and GTP
Differential receptors create patterns diagnostic for ATP and GTP
复制标题
DOI：
10.1021/ja021230b
复制
发表时间：
2003-02-05
影响因子：
15
通讯作者：
Anslyn, EV
中科院分区：
文献类型：
--
作者：
McCleskey, SC;Griffin, MJ;Anslyn, EV
在此，我们报告了树脂结合传感器库与多组件传感器阵列的组合。这种新颖的组合阵列传感器系统显示出对溶液中的核苷酸磷酸盐的选择性。锚定受体的设计包括与肽库偶联的 1,3,5-三取代-2,4,6-三乙苯支架。每个化学传感器都被放置在硅晶片内的微机械腔中，电荷耦合设备观察到的光学变化导致溶液的近实时数字分析。进行了比色位移测定，并对选定的传感整体进行了时间依赖性成像研究，结果在添加腺苷 5'-三磷酸 (ATP)、腺苷 5'-单磷酸 (AMP) 或鸟苷 5'-三磷酸 (GTP) 时产生差异响应。这种方法的一个优点是它创建了一个传感器阵列，可以为每种分析物提供指纹响应。主成分分析表明化学传感器库可以区分 ATP、GTP 和 AMP。根据因子加载值，对库中的各个传感器进行测序以阐明其化学成分。
Herein we report the combination of a library of resin-bound sensors along with a multicomponent sensor array. This novel combinatorial array sensor system shows selectivity for nucleotide phosphates in solution. The design of the anchored receptor includes a 1,3,5-trisubstituted-2,4,6-triethylbenzene scaffold coupled with peptide libraries. Each chemosensor is placed into a micromachined cavity within a silicon wafer, and the optical changes observed by a charged-coupled device result in near-real-time digital analysis of solutions. A colorimetric displacement assay was performed, and time-dependent imaging studies of the selected sensing ensembles result in a differential responses upon addition of adenosine 5‘-triphosphate (ATP), adenosine 5‘-monophosphate (AMP), or guanosine 5‘-triphosphate (GTP). An advantage to this approach is that it creates an array of sensors that gives a fingerprint response for each analyte. Principal component analysis indicates that the library of chemosensors can differentiate between ATP, GTP, and AMP. On the basis of factor loading values, individual sensors from the library were sequenced to elucidate their chemical composition.