In situ observation of cellular organelles with a contact x-ray microscop
In situ observation of cellular organelles with a contact x-ray microscop
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使用接触式 X 射线显微镜原位观察细胞器
DOI：
10.1088/1742-6596/463/1/012056
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
K Yasuda and K Shinohara
中科院分区：
文献类型：
--
作者：
M Kado;M Kishimoto;S Tamotsu;K Yasuda and K Shinohara
已开发出与高强度激光等离子体软 X 射线源相结合的接触式 X 射线显微镜，并可对细胞器进行原位观察。软X射线是通过将高功率激光脉冲照射到薄箔金靶上而产生的，获得约1.3×10 15 光子/sr，这使得活体湿生物细胞的内部结构能够被成像。单次闪光成像是对活体生物细胞的细胞器进行成像的关键技术，避免由于运动模糊和辐射损伤而导致图像空间分辨率下降。将荧光显微镜与软 X 射线显微镜结合使用来识别细胞器，可以在软 X 射线图像中精确识别多种细胞器。荧光显微镜与软 X 射线显微镜的结合将非常有用，并将使该技术成为分析生物细胞活功能的强大工具。
A contact x-ray microscope coupled with a highly intense laser plasma soft x-ray source has been developed and in situ observations of cellular organelles have been conducted. The soft x-rays were generated by irradiating a high power laser pulse onto a thin-foiled gold target and about 1.3× 10 15 photons/sr were obtained, which allowed the inner structures of live wet biological cells to be imaged. Single shot flash imaging is a key technique to image cellular organelles of live biological cells avoiding degradation of the spatial resolution of the images resulting from motion blur and radiation damage. The use of a fluorescence microscope to identify cellular organelles in conjunction with the soft x-ray microscope has allowed several cellular organelles to be identified precisely in the soft x-ray images. Combining the fluorescence microscope with the soft x-ray microscope will be very useful and will establish the technique as a powerful tool to analyze living function of biological cells.